Presentation Information
[P02-276]Exploring Compatibility for Dual-Carbon PHB Production in Cupriavidus necator
○ALIYAH ALIYAH1 (1. Kobe University (Japan))
Keywords:
Cuprividus necator,PHB,Dual Carbon,Metabolomics
The use of formate as an alternative carbon and energy source has attracted growing interest for sustainable bioproduction due to its potential generation from CO2 via electrochemical processes, supporting carbon recycling. However, when used as a sole substrate, formate often limits biomass growth and polyhydroxybutyrate (PHB) production because it cannot fully support cellular biosynthesis. This study aims to address this limitation by evaluating a dual-carbon strategy combining formate with additional carbon sources to improve PHB production performance.
In this approach, formate functions as an auxiliary energy source supplying reducing power, while co-substrates provide carbon skeletons for biomass formation and PHB biosynthesis. Fructose and acetate were selected as co-substrates due to their distinct metabolic roles, and the system was further analyzed using metabolomic profiling to assess intracellular responses.
Experimental observations indicated that formate was preferentially consumed, reflecting its role in meeting cellular energy demands. The addition of formate enhanced biomass and PHB accumulation in acetate-based cultures compared to acetate alone, whereas no comparable improvement was observed in fructose-based systems, indicating substrate-dependent compatibility. These findings suggest that the effectiveness of dual-carbon strategies is governed by the metabolic compatibility between substrates rather than their individual assimilation efficiency.
Overall, this study highlights acetate–formate compatibility as a promising strategy for improving PHB production and provides a basis for designing more efficient and sustainable bioprocesses.
In this approach, formate functions as an auxiliary energy source supplying reducing power, while co-substrates provide carbon skeletons for biomass formation and PHB biosynthesis. Fructose and acetate were selected as co-substrates due to their distinct metabolic roles, and the system was further analyzed using metabolomic profiling to assess intracellular responses.
Experimental observations indicated that formate was preferentially consumed, reflecting its role in meeting cellular energy demands. The addition of formate enhanced biomass and PHB accumulation in acetate-based cultures compared to acetate alone, whereas no comparable improvement was observed in fructose-based systems, indicating substrate-dependent compatibility. These findings suggest that the effectiveness of dual-carbon strategies is governed by the metabolic compatibility between substrates rather than their individual assimilation efficiency.
Overall, this study highlights acetate–formate compatibility as a promising strategy for improving PHB production and provides a basis for designing more efficient and sustainable bioprocesses.
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