Presentation Information
[P03-383]A Lrp/AsnC-type transcriptional regulator in Archaea
○Rengwei Liu1, Ryo Maruyama1, Yasuyuki Yamamoto1, Mitsuru Tanimoto1, Yuta Michimori1, Tamotsu Kanai1, Haruyuki Atomi1 (1. Kyoto University (Japan))
Keywords:
Archaea,metabolism,regulation,transcription
The Lrp or Lrp/AsnC family of transcriptional regulators are widespread in many bacterial species, where they are generally involved in controlling gene expression in response to nutritional signals, particularly amino acids. The Lrp protein in Escherichia coli is a well-studied example, which is known to regulate more than one third of the genes on the E. coli genome. Studies in bacterial Lrp/AsnC family regulators revealed that this family of regulators plays an important role in global transcription regulation. Genome sequences shows that Lrp/AsnC homologs are present in almost all archaeal species. As the basal transcriptional machinery in archaea differ to that in bacteria and instead resemble that of eukaryotes, the functions and mechanisms of archaeal Lrp/AsnC-type regulators may differ to the previously characterized Lrp/AsnC family regulators from bacteria.
The hyperthermophilic archaeon Thermococcus kodakarensis is an obligate anaerobe and heterotroph, utilizing a variety of organic compounds including amino acids, peptides and polysaccharides. We have been studying many aspects of metabolism and its regulation in this organism, including the catabolism and biosynthesis of sugars, amino acids, nucleic acids and cofactors. This study examines a Lrp/AsnC-type regulator in a hyperthermophilic archaeon, Thermococcus kodakarensis. Through gene disruption, it was observed that loss of this regulator perturbs the transcript levels of several genes, implicating it is involved in metabolic regulation of amino acids. Biochemical analyses confirmed that this regulator binds specifically to the promoter regions of its target genes. Furthermore, its DNA-binding behavior and oligomeric state were found to be dynamically influenced by amino acid concentration.
This work provides insight into the understanding of archaeal transcriptional regulators and their functions in metabolic networks in response to environmental and nutritional cues. As homologs of this regulator are conserved in many archaea, these findings offer a framework for understanding similar regulatory mechanisms in other archaeal species.
The hyperthermophilic archaeon Thermococcus kodakarensis is an obligate anaerobe and heterotroph, utilizing a variety of organic compounds including amino acids, peptides and polysaccharides. We have been studying many aspects of metabolism and its regulation in this organism, including the catabolism and biosynthesis of sugars, amino acids, nucleic acids and cofactors. This study examines a Lrp/AsnC-type regulator in a hyperthermophilic archaeon, Thermococcus kodakarensis. Through gene disruption, it was observed that loss of this regulator perturbs the transcript levels of several genes, implicating it is involved in metabolic regulation of amino acids. Biochemical analyses confirmed that this regulator binds specifically to the promoter regions of its target genes. Furthermore, its DNA-binding behavior and oligomeric state were found to be dynamically influenced by amino acid concentration.
This work provides insight into the understanding of archaeal transcriptional regulators and their functions in metabolic networks in response to environmental and nutritional cues. As homologs of this regulator are conserved in many archaea, these findings offer a framework for understanding similar regulatory mechanisms in other archaeal species.
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