[P005] Poster

12:10 PM - 1:00 PM JST(3:10 AM - 4:00 AM UTC)

[P03-419] Electroactive Carbon Nanofiber Layers for Effective Osteodifferentiation

○Elif Beyza Demiray¹, Selestin Raja Iruthayapandi², Hee Jeong Jang², Dongwoo Gi³, Bongju Kim⁴, Seon Yeong Chae⁵, Dong-Wook Han^1,2,3 (1. Department of Cogno-Mechatronic Engineering, Pusan National University (Korea), 2. Institute of Nano-Bio Convergence, Pusan National University (Korea), 3. School of Transdisciplinary Engineering, Pusan National University (Korea), 4. Seoul National University Dental Hospital (Korea), 5. Engineering Research Center for Color-Modulated Extra-Sensory Perception Technology (Korea))

Keywords:

Pyrolytically stripped carbon nanofiber,electroactive scaffold,MC3T3-E1 preosteoblasts,bone tissue regeneration

Non-woven nanofiber mats, with high permeability, nutrient diffusivity, and mechanical robustness, are widely used as scaffolds for tissue engineering. Carbon nanofibers (CNF) are often incorporated into biomaterials to enhance electroactivity and mechanical strength, yet their direct use as scaffolds remains underexplored. Here, we report the fabrication of CNF-coated polycaprolactone (PCL) nanofiber mats (CNF@PCL) via drop-casting of pyrolytically stripped CNF. Morphological analysis confirmed uniform CNF deposition, producing a nanofibrous surface architecture. CNF@PCL exhibited dramatically enhanced electrical conductivity, four orders of magnitude higher than pristine PCL. MC3T3-E1 preosteoblasts proliferated comparably on both scaffolds, whereas CNF@PCL significantly promoted early osteogenic differentiation, as indicated by elevated ALP activity at day 14 and robust mineralized calcium deposition. These results demonstrate that electroactive CNF matrices can functionally mimic electrospun nanofibers, offering a promising platform for bone tissue regeneration and motivating further in vivo validation.