Presentation Information
[P03-431]Customizable Assemblable 3D-Printed Platforms for Site-Specific Tissue Regeneration
○Seunghun S. Lee1, Inseon Kim1 (1. Dongguk University (Korea))
Keywords:
3D Printing,Scaffold,Assmebly,Patient-specific,Tissue Engineering
[Purpose]
Current additive manufacturing for patient-specific implants is labor-intensive and financially burdensome. This study aims to develop a modular, easy-to-assemble scaffold system that allows surgeons to intuitively create bespoke implants on-site while enabling localized, site-specific regenerative treatments.
[Method]
A digital light processing (DLP) technique was utilized to fabricate a hive-structured assemblable scaffold (HIVE). This platform functions as a customizable carrier for microcryogels (MCs) loaded with diverse biological factors—such as growth factors (GF), bioceramics, or cells—within distinct structural pockets. The system's therapeutic efficacy was evaluated in vitro using human mesenchymal stem cells (hMSCs) and human umbilical vein endothelial cells (HUVECs), followed by an in vivo rat subcutaneous implantation study.
[Results]
The HIVE platform demonstrated controlled release of BMP-2 and VEGF, successfully inducing targeted osteogenesis and angiogenesis. Bioceramic-loaded MCs enhanced mineralization, while HUVEC-loaded MCs upregulated osteogenic and angiogenic gene expression in hMSCs. Distinct local effects and successful cell migration were confirmed across various MC combinations. The in vivo implantation validated the platform's capability to drive site-specific osteogenesis and angiogenesis.
[Consideration]
The HIVE system provides exceptional modular flexibility, allowing unlimited structural combinations to accommodate specific defect geometries. By compartmentalizing different therapeutic agents within a single construct, it ensures precise spatial control over the tissue regeneration process, overcoming the limitations of uniform bulk scaffolds.
[Conclusion]
The assemblable HIVE platform offers a highly versatile and cost-effective solution for both patient- and site-specific treatments. It demonstrates significant clinical potential, particularly for advanced bone tissue engineering applications.
Current additive manufacturing for patient-specific implants is labor-intensive and financially burdensome. This study aims to develop a modular, easy-to-assemble scaffold system that allows surgeons to intuitively create bespoke implants on-site while enabling localized, site-specific regenerative treatments.
[Method]
A digital light processing (DLP) technique was utilized to fabricate a hive-structured assemblable scaffold (HIVE). This platform functions as a customizable carrier for microcryogels (MCs) loaded with diverse biological factors—such as growth factors (GF), bioceramics, or cells—within distinct structural pockets. The system's therapeutic efficacy was evaluated in vitro using human mesenchymal stem cells (hMSCs) and human umbilical vein endothelial cells (HUVECs), followed by an in vivo rat subcutaneous implantation study.
[Results]
The HIVE platform demonstrated controlled release of BMP-2 and VEGF, successfully inducing targeted osteogenesis and angiogenesis. Bioceramic-loaded MCs enhanced mineralization, while HUVEC-loaded MCs upregulated osteogenic and angiogenic gene expression in hMSCs. Distinct local effects and successful cell migration were confirmed across various MC combinations. The in vivo implantation validated the platform's capability to drive site-specific osteogenesis and angiogenesis.
[Consideration]
The HIVE system provides exceptional modular flexibility, allowing unlimited structural combinations to accommodate specific defect geometries. By compartmentalizing different therapeutic agents within a single construct, it ensures precise spatial control over the tissue regeneration process, overcoming the limitations of uniform bulk scaffolds.
[Conclusion]
The assemblable HIVE platform offers a highly versatile and cost-effective solution for both patient- and site-specific treatments. It demonstrates significant clinical potential, particularly for advanced bone tissue engineering applications.
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