[P005] Poster

12:10 PM - 1:00 PM JST(3:10 AM - 4:00 AM UTC)

[P03-437] Bioactive Fucoidan from the Brown Seaweed Turbinaria decurrens Efficiently Produced by a Microwave-Assisted Extraction Process

○Ellya Sinurat¹, Raden Syabila Anandyta², Hari Eko Irianto¹, Eko Setio Wibowo¹, Yulia Anita¹, Sosaku Ichikawa³ (1. National Research and Innovation Agency, Indonesia (Indonesia), 2. Pancasila University, Indonesia (Indonesia), 3. University of Tsukuba, Japan (Japan))

Keywords:

Marine polysaccharides,Fucoidan,Microwave-assisted extraction,Turbinaria decurrens,Sustainable marine bioprocessing

Marine bioresources represent a critical frontier in blue biotechnology, offering sustainable sources of high-value biomolecules for industrial and biomedical applications. Fucoidan, a sulfated polysaccharide derived from brown seaweeds, has emerged as a promising marine biopolymer due to its diverse bioactivities and functional properties. This study aimed to optimize the extraction of fucoidan from the underutilized tropical brown seaweed Turbinaria decurrens in Indonesian coastal waters and evaluate its physicochemical characteristics and biofunctional potential. A green and efficient microwave-assisted extraction (MAE) approach was employed and optimized using response surface methodology (RSM) based on a Box-Behnken design (BBD). The effects of key bioprocessing parameters microwave power (200-700 W), extraction time (5-30 min), and solvent-to-solid ratio (10-50 mL/g water) were systematically investigated in relation to fucoidan yield, total fucose, and sulfate content. The raw biomass exhibited suitable processing characteristics, including moisture content of 11.32%, ash content of 9.68%, and near-neutral pH (6.0), supporting its potential as a stable marine feedstock. The optimized extraction conditions predicted by RSM (44.5 mL/g, 24.5 min, 495.5 W) resulted in a fucoidan yield of 14.44%, with enhanced fucose (5.2%) and sulfate (2.0%) contents, confirming both model reliability and preservation of key structural features. These results demonstrate the effectiveness of MAE as a rapid and scalable marine bioprocessing strategy. Biofunctional evaluation revealed that the extracted fucoidan exhibited strong antioxidant activity (ABTS IC₅₀ = 50.7 µg/mL), moderate antibacterial activity against Helicobacter pylori, and limited a-glucosidase inhibitory activity (IC₅₀ > 500 µg/mL). The observed bioactivities highlight the importance of structural attributes, particularly sulfate content, in determining functional performance. This study provides the first systematic optimization of microwave-assisted fucoidan extraction from T. decurrens, positioning this species as a novel and underexploited tropical marine bioresource. The integration of MAE and RSM offers a sustainable, time-efficient, and scalable extraction platform that aligns with the principles of blue biotechnology and marine resource valorization. Collectively, these findings support the development of value-added marine bioproducts and contribute to advancing the sustainable bioeconomy based on tropical seaweed resources. Acknowledgements: This research was supported by JST-JICA SATREPS, Japan (JPMJSA2307)