[P006] Poster

1:00 PM - 1:50 PM JST(4:00 AM - 4:50 AM UTC)

[P03-314] Genome-wide identification of genetic regulatory elements in Streptomyces tsukubaensis NRRL 18488

○Woori Kim^1,2, Namil Lee^1,3,4,5,12, Ji Hun Kim^1,2, Yongjae Lee^1,2, Soonkyu Hwang^1,2, Gahyeon Kim^1,2, Hyeseong Kim^1,2, Qingyun Dan^4,5, Matthias Schmidt^3,4,5, Yeo Joon Yoon⁶, Suhyung Cho^1,2, Bernhard Palsson^7,8,9, Jay D. Keasling^{3,4,5,7,10,11}, Byung-Kwan Cho^2,12 (1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon (Korea), 2. KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon (Korea), 3. California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, California (USA), 4. Joint BioEnergy Institute, Emeryville, California (USA), 5. Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California (USA), 6. College of Pharmacy, Seoul National University, Seoul (Korea), 7. Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby (Denmark), 8. Department of Bioengineering, University of California San Diego, La Jolla, California (USA), 9. Department of Pediatrics, University of California San Diego, La Jolla, California (USA), 10. Department of Chemical & Biomolecular Engineering, University of California, Berkeley, California (USA), 11. Department of Bioengineering, University of California, Berkeley, California (USA), 12. Graduate School of Engineering Biology, Korea Advanced Institute of Science and Technology, Daejeon (Korea))

Keywords:

Streptomyces,Secondary metabolism,FK506,Transcription unit

Streptomyces species are well known for their ability to produce a diverse array of secondary metabolites, many of which are utilized in clinical applications. Among them, Streptomyces tsukubaensis NRRL 18488 is an industrially significant strain due to its production of the immunosuppressant FK506. Understanding the complex regulation of secondary metabolism requires a comprehensive analysis of the primary transcriptome architecture, which elucidates transcriptional and post-transcriptional regulatory elements. In this study, we identified a total of 2,389 transcription start sites and 2,110 transcript end positions, which were integrated to define 1,225 transcription units (TUs), including numerous non-coding TUs. Notably, within the FK506 biosynthetic gene cluster, we uncovered previously uncharacterized post-transcriptional regulation governing the production of allylmalonyl-CoA, an essential precursor for FK506 biosynthesis. This genome-wide analysis provides a detailed understanding of the complex regulatory mechanisms orchestrating secondary metabolism in Streptomyces. This research was supported by a grant of the Korea-US Collaborative Research Fund (KUCRF), funded by the Ministry of Science and ICT and Ministry of Health & Welfare, Republic of Korea (RS-2024-00468410 to B.-K.C.), by the KAIST Strategic Research Program (N10260074), and by the KAIST Jang Young Sil Fellow Program.