Presentation Information
[P03-364]Development of functional metabolic cocktail to promote production of useful compounds in soybean roots
○Hiromitsu Tabeta1,2, Masami Hirai2 (1. Grad. Sch. Agr. Sci., Kobe Univ. (Japan), 2. RIKEN CSRS (Japan))
Keywords:
Plant Metabolomics,Isofllavone biosynthesis
[Objective]
Reducing the environmental burden of agriculture is essential for achieving sustainable crop production. There is an urgent need to develop technologies that reduce fertilizer and water inputs per unit yield while maintaining crop productivity. We previously identified an organic acid, LAPA, as a functional metabolite that enhances nutrient uptake efficiency. In soybean, LAPA application was shown to induce morphological changes in roots and promote the biosynthesis of beneficial metabolites, potentially through modulation of the endogenous glutamate (Glu) / arginine (Arg) balance. Based on these findings, this study aimed to develop a metabolic cocktail containing LAPA and amino acids to further enhance the production of beneficial metabolites in soybean roots.
[Methods]
Soybean roots were treated with LAPA in combination with multiple amino acids. To evaluate the metabolic effects of these treatments, wide-target metabolomic analysis was performed on soybean roots. Changes in the accumulation of industrially relevant compounds and plant specialized metabolites were assessed, and the effects of different amino acid combinations were compared to identify the most effective metabolic cocktail.
[Results]
Treatment with the metabolic cocktail promoted the biosynthesis of adipic acid, an industrially useful compound, in soybean roots. In addition, the accumulation of isoflavones, a major class of plant specialized metabolites, was enhanced. Among the combinations tested, the cocktail containing LAPA and Glu (“LG cocktail”) showed the strongest effect on metabolic alteration and was identified as the most effective treatment for promoting the production of these metabolites.
[Discussion]
Because many plants specialized metabolites are synthesized from amino acids, the observed enhancement of metabolite production may be associated with changes in amino acid balance induced by LAPA and amino acid supplementation. In particular, modulation of the endogenous Glu/Arg balance may contribute to the increased biosynthesis of beneficial compounds in soybean roots. Furthermore, since isoflavones exuded into the rhizosphere are known to promote microbiome formation, the LG cocktail may influence not only metabolic activity in roots but also rhizosphere function and plant performance.
[Conclusion]
These results demonstrate that supplementation with LAPA and amino acids can enhance the production of beneficial metabolites in soybean roots. In particular, the LG cocktail was identified as the most effective combination for promoting the biosynthesis of adipic acid and isoflavones. This metabolic cocktail may serve as a promising strategy for improving root functional traits and potentially supporting plant growth under sustainable agricultural production systems.
Reducing the environmental burden of agriculture is essential for achieving sustainable crop production. There is an urgent need to develop technologies that reduce fertilizer and water inputs per unit yield while maintaining crop productivity. We previously identified an organic acid, LAPA, as a functional metabolite that enhances nutrient uptake efficiency. In soybean, LAPA application was shown to induce morphological changes in roots and promote the biosynthesis of beneficial metabolites, potentially through modulation of the endogenous glutamate (Glu) / arginine (Arg) balance. Based on these findings, this study aimed to develop a metabolic cocktail containing LAPA and amino acids to further enhance the production of beneficial metabolites in soybean roots.
[Methods]
Soybean roots were treated with LAPA in combination with multiple amino acids. To evaluate the metabolic effects of these treatments, wide-target metabolomic analysis was performed on soybean roots. Changes in the accumulation of industrially relevant compounds and plant specialized metabolites were assessed, and the effects of different amino acid combinations were compared to identify the most effective metabolic cocktail.
[Results]
Treatment with the metabolic cocktail promoted the biosynthesis of adipic acid, an industrially useful compound, in soybean roots. In addition, the accumulation of isoflavones, a major class of plant specialized metabolites, was enhanced. Among the combinations tested, the cocktail containing LAPA and Glu (“LG cocktail”) showed the strongest effect on metabolic alteration and was identified as the most effective treatment for promoting the production of these metabolites.
[Discussion]
Because many plants specialized metabolites are synthesized from amino acids, the observed enhancement of metabolite production may be associated with changes in amino acid balance induced by LAPA and amino acid supplementation. In particular, modulation of the endogenous Glu/Arg balance may contribute to the increased biosynthesis of beneficial compounds in soybean roots. Furthermore, since isoflavones exuded into the rhizosphere are known to promote microbiome formation, the LG cocktail may influence not only metabolic activity in roots but also rhizosphere function and plant performance.
[Conclusion]
These results demonstrate that supplementation with LAPA and amino acids can enhance the production of beneficial metabolites in soybean roots. In particular, the LG cocktail was identified as the most effective combination for promoting the biosynthesis of adipic acid and isoflavones. This metabolic cocktail may serve as a promising strategy for improving root functional traits and potentially supporting plant growth under sustainable agricultural production systems.
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