[P006] Poster

1:00 PM - 1:50 PM JST(4:00 AM - 4:50 AM UTC)

[P03-446] Evaluation of the Tomato spotted wilt virus(TSWV)-based Vectors for Gene Editing in Petunia

○Yu,Qin Sun¹, Chien-Hung Chung¹, Hsin-Mei Ku¹ (1. NATIONAL CHUNG HSING UNIVERSITY (Taiwan))

Keywords:

Petunia hybrida,virus-induced gene editing (VIGE),FLORAL BINDING PROTEIN6 (FBP6),Tomato spotted wilt virus (TSWV),Streptococcus pyogenes Cas9 (spCas9)

Petunia (Petunia hybrida) is a popular ornamental plant in horticulture. Due to the limitations of traditional gene editing approaches, such as time, labor intensity, low efficiency, and cost, this study aims to test a simple and efficient virus-induced gene editing (VIGE) approach using Tomato spotted wilt virus (TSWV) as a viral vector, which has a broad host range, including petunia. A gRNA targeting the petunia FLORAL BINDING PROTEIN6 (FBP6) gene, a key regulator of the double-flower, was designed and constructed into the TSWV-VIGE vector, which harbors the Streptococcus pyogenes Cas9 (spCas9) gene. The TSWV-VIGE vectors were agroinfiltrated into the leaves of a single-flower petunia cultivar. Leaves were harvested 7–14 days post-inoculation for sequence analysis. A -18 base deletion near the Protospacer Adjacent Motif (PAM) of the target region was detected, confirming the feasibility of applying TSWV-VIGE in petunia.