Presentation Information
[P03-450]Plant Extracellular Vesicles as a Platform for Bioactive Molecule Delivery
○Yury Shkryl1, Maria Sorokina1, Alexandra Fialko1, Olga Grishchenko1, Yulia Yugay1 (1. Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences (Russia))
Keywords:
extracellular vesicles,plant cell cultures,bioactive compounds,trans-δ-viniferin,artificial microRNAs
Extracellular vesicles (EVs) are nano-sized membrane structures secreted by cells and involved in intercellular communication. They transport proteins, lipids, metabolites, and nucleic acids, enabling the transfer of biologically active molecules between cells and across kingdoms. Plant cell cultures provide a controlled and scalable system for EV production, yet remain underexplored. Compared to mammalian EVs, they offer advantages such as low immunogenicity and the use of renewable sources. In addition, they ensure uniformity, sterility, and reduced environmental variability, while allowing targeted manipulation of metabolic pathways and experimental conditions.
In our work, we investigated EVs derived from callus cultures of grapevine (Vitis vinifera). The isolated EVs were characterized using a combination of electron microscopy, nanoparticle tracking analysis, and molecular markers. Our results indicate that grape callus-derived EVs exhibit typical EV morphology and size distribution, while also showing specific features potentially associated with plant-specific biogenesis pathways. In particular, our data suggest alterations in components related to EVs formation, consistent with a non-canonical or modified ESCRT-associated mechanism. Compositional analysis revealed that these EVs are enriched in secondary metabolites, including stilbenoids such as trans-δ-viniferin derivatives, as well as stress-related proteins. Functional assays demonstrated that the EVs reduced viability of the MDA-MB-231 cell line in a dose-dependent manner, while showing minimal effects on HEK293 cells. The treatment induced G1 phase arrest and apoptosis in MDA-MB-231 cells.
Importantly, we explored strategies for functionalization of plant EVs. Using classical approaches, we demonstrated that elicitation of secondary metabolism (e.g., methyl jasmonate, salicylic acid, chitosan) can modulate the cargo composition of EVs, enhancing the accumulation of target metabolites. In parallel, we investigated genetic engineering approaches to direct the loading of specific molecules into EVs. As a proof of concept, we demonstrated the delivery of engineered microRNA targeting GFP, confirming the feasibility of programming EV cargo through transgene expression.
Overall, our results highlight plant cell culture-derived EVs as a versatile and tunable platform for the production of bioactive nanocarriers. The combination of controlled cultivation systems, metabolic engineering, and molecular tools opens new avenues for the development of EV-based therapeutics, cosmetic ingredients, and delivery systems.
Financial support was provided by the Russian Science Foundation, Grant no. 25-24-00996 (Shkryl Y.N.).
In our work, we investigated EVs derived from callus cultures of grapevine (Vitis vinifera). The isolated EVs were characterized using a combination of electron microscopy, nanoparticle tracking analysis, and molecular markers. Our results indicate that grape callus-derived EVs exhibit typical EV morphology and size distribution, while also showing specific features potentially associated with plant-specific biogenesis pathways. In particular, our data suggest alterations in components related to EVs formation, consistent with a non-canonical or modified ESCRT-associated mechanism. Compositional analysis revealed that these EVs are enriched in secondary metabolites, including stilbenoids such as trans-δ-viniferin derivatives, as well as stress-related proteins. Functional assays demonstrated that the EVs reduced viability of the MDA-MB-231 cell line in a dose-dependent manner, while showing minimal effects on HEK293 cells. The treatment induced G1 phase arrest and apoptosis in MDA-MB-231 cells.
Importantly, we explored strategies for functionalization of plant EVs. Using classical approaches, we demonstrated that elicitation of secondary metabolism (e.g., methyl jasmonate, salicylic acid, chitosan) can modulate the cargo composition of EVs, enhancing the accumulation of target metabolites. In parallel, we investigated genetic engineering approaches to direct the loading of specific molecules into EVs. As a proof of concept, we demonstrated the delivery of engineered microRNA targeting GFP, confirming the feasibility of programming EV cargo through transgene expression.
Overall, our results highlight plant cell culture-derived EVs as a versatile and tunable platform for the production of bioactive nanocarriers. The combination of controlled cultivation systems, metabolic engineering, and molecular tools opens new avenues for the development of EV-based therapeutics, cosmetic ingredients, and delivery systems.
Financial support was provided by the Russian Science Foundation, Grant no. 25-24-00996 (Shkryl Y.N.).
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