[P007] Poster

12:10 PM - 1:00 PM JST(3:10 AM - 4:00 AM UTC)

[P04-489] Anti-diabetic and Anti-Inflammatory Potential of Brown Seaweeds from the Banten Coastal Area Indonesia

○Setyani Budiari¹, Zhe Yang¹, Chika Takumi², Sri Ayu Lestari³, Ayase Hoshino¹, Hakiki Melanie⁴, Ellya Sinurat⁵, Ambo Tuwo⁶, Sosaku Ichikawa⁷, Yusaku Miyamae⁷ (1. Master’s/Doctoral Program in Life Science Innovation, Graduate School of Sciences and Technology, University of Tsukuba, Ibaraki 305-8577, Japan (Japan), 2. Master’s Program in Agro-Bioresources Science and Technology, Graduate School of Science and Technology, University of Tsukuba, Ibaraki 305-8577, Japan (Japan), 3. Doctoral Program in Fisheries Science, Faculty of Marine Sciences and Fisheries, Hasanuddin University, Makassar 90245, Indonesia (Indonesia), 4. Research Center for Molecular Chemistry, National Research and Innovation Agency (BRIN), Banten 15314, Indonesia (Indonesia), 5. Research Center for Biomass and Bioproducts, National Research and Innovation Agency (BRIN), Banten 15314, Indonesia (Indonesia), 6. Multitrophic Research Group, Faculty of Marine Sciences and Fisheries, Hasanuddin University, Makassar 90245, Indonesia (Indonesia), 7. Institute of Life and Environmental Science, University of Tsukuba, Ibaraki 305-8572, Japan (Japan))

Keywords:

Indonesia Brown seaweeds,α-glucosidase inhibition,nitric oxide inhibition

Indonesia is an archipelagic country characterized by abundant marine biodiversity, including diverse brown seaweed resources that are potential sources of bioactive compounds. Indonesian brown seaweeds are utilized to produce some downstream products such as alginate, cellulose materials, fucoidan, and fertilizers. However, the potential of their bioactive compounds remains underexplored. The majority of brown seaweeds in Indonesia are still collected from wild populations, and a further evaluation of their bioactive properties could open new opportunities for their sustainable utilization in food, nutraceutical, and pharmaceutical applications, as well as support future cultivation. The coastal waters of Banten harbour a significant habitat of brown seaweeds in Indonesia and may provide a valuable source of bioactive compounds. Diabetes mellitus (DM) is a metabolic disorder with increasing global prevalence, and its development is closely associated with inflammation. During chronic inflammation, activation of inflammatory pathways can lead to the release of pro-inflammatory cytokines such as IL-1β, IL-6, and TNF-α, finally leading to insulin resistance and β-cell dysfunction. This mechanism highlights the necessity to identify natural compounds with potential antidiabetic and anti-inflammatory activities, including those derived from seaweed. Therefore, this study aims to evaluate the potential of two brown seaweed species from the Banten coastal area of Indonesia (Sargassum sp. and Padina sp.) as natural sources of antidiabetic and anti-inflammatory activities. Antidiabetic potential was assessed using an α-glucosidase inhibition assay on crude ethanolic, ethyl acetate, and n-hexane extracts obtained through maceration. The ethanolic extract of Sargassum sp. and the ethyl acetate extract of Padina sp. exhibited significant α-glucosidase inhibitory activity (>90%) at a concentration of 0.25 mg/mL, and were further analyzed using GC-MS. The GC-MS profiles revealed that the selected extracts were primarily composed of long-chain fatty acids and their derivatives, such as n-hexadecanoic acid and tetradecanoic acid. Furthermore, terpenoid compounds such as loliolide, neophytadiene, and phytol were also identified and predicted to contribute to α-glucosidase inhibitory activity based on literature reports. Anti-inflammatory activity was evaluated in vitro using crude methanolic extracts by measuring inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-induced RAW264.7 macrophage-like cells, along with a cell viability assay (MTT). All two species showed a significant reduction in NO production without a decrease in cell viability (>80%) at concentrations ranging from 1 µg/mL to 50 µg/mL. Stepwise fractionation was performed by liquid-liquid partitioning, yielding four fractions (hexane, chloroform, ethyl acetate, and water) for Sargassum sp. and Padina sp. The hexane fractions of Sargassum sp. and Padina sp. showed the most significant inhibition of NO production. ¹H-NMR analysis of the hexane fraction suggested the presence of terpenoid compounds in Sargassum sp. and glycolipid-related compounds in Padina sp. These findings demonstrate that brown seaweeds from the Banten coastal area exhibit promising bioactivities and potential as functional ingredients derived from local marine resources. This research was supported by JST-JICA SATREPS, Japan (JPMJSA2307).