[P007] Poster

12:10 PM - 1:00 PM JST(3:10 AM - 4:00 AM UTC)

[P04-525] Application of Large-Pore Cellulose Chromatography Media: Cellufine MLP for High-Performance Viral Vector and Vaccine Purification

○Junya Toba¹, Ayana Uehara¹, Chigusa Mori¹, Yoshihiro Matsumoto¹, Eri Iwamoto¹ (1. JNC corporation (Japan))

Keywords:

Chromatography,Viral vector,Biopharmaceuticals,Porous particle,Convective transport

[Purpose]
The aim was to develop chromatography media capable of efficiently purifying large biomolecules, such as viral vectors and whole virus vaccines, which are increasingly important in biotherapeutic applications.
[Method]
We created Cellufine MLP^TM, a new class of permeable cellulose beads with continuous pore structures over 1 μm within 90 μm spherical particles. These beads were evaluated for purification of large biomolecules using application studies with inactivated vaccine and gene therapy vectors.
[Results]
Cellufine MLP^TM demonstrated high binding capacity, improved recovery, and enhanced impurity removal compared to conventional media. The unique large-pore architecture enabled efficient capture and processing of virus-sized particles.
[Consideration]
The observed improvements in binding capacity and impurity removal can be attributed to the continuous large-pore structure of Cellufine MLP^TM beads, which facilitates convective transport within the particle interior. This architectural feature mitigates the diffusional limitations typically associated with conventional chromatography media, allowing for more effective processing of virus-sized biomolecules.
[Conclusion]
These findings suggest that the transition from diffusion-dominated to convection-dominated transport enabled by the large-pore cellulose matrix offers clear advantages for the purification of large biomolecules. Cellufine MLP^TM may contribute to improved process efficiency and scalability in biotherapeutic manufacturing, particularly in the context of viral vectors, vaccines, and similarly sized modalities.