Presentation Information
[P04-585]The Taiwan Mouse Clinic: the first user-friendly platform for high-quality, high-throughput, comprehensive phenotyping and drug testing of mouse models in Asia
○Chao-Kuen Lai1,2, Wei–Wei Chiang1,2, I–Chi Yang1,3, Yu-Chang Chen1,2, Yu-Fang Lin1,3, Wan–Chen Chao1,3, Yan–Zhen Liu1,3, Cheng-Wei Lo1,3, Hsiao–Han Hung1,2, Chih-Cheng Chen1,2,3 (1. Taiwan Mouse Clinic–National Comprehensive Mouse Phenotyping and Drug Testing Center, Academia Sinica (Taiwan), 2. Biomedical Translation Research Center, Academia Sinica (Taiwan), 3. Institute of Biomedical Sciences, Academia Sinica (Taiwan))
Keywords:
Taiwan Mouse Clinic,mouse phenotyping,drug testing,disease model,safety pharmacology
[Purpose]
The Taiwan Mouse Clinic (TMC) is a specific pathogen-free (SPF) animal facility designed for comprehensive mouse phenotyping and drug testing, as well as specialized, customized services in disease model studies and exploratory safety pharmacology.
[Method]
To facilitate user-friendly services, TMC has developed a unique and straightforward Standard Operating Procedure (SOP) for the easy check-in of mice. This SOP allows researchers' SPF mice to be admitted directly into the facility without a quarantine period. In addition, mice are housed in separate, individually ventilated cages (IVC) carts or rooms, based on user groups, to ensure proper health monitoring and care. This practice has helped TMC maintain an 18-year record free of 12 specific pathogens. Notably, TMC has developed a highly effective method for “cleaning” mice that circumvents the need for re-derivation through embryo transfer or cesarean hysterectomy. In-depth studies have shown that environmental effects and assay interactions would affect mouse phenotyping.
[Results]
We found that assay interactions and environmental factors significantly influence mouse phenotyping, particularly the effects of phenotyping pipelines and bedding type on behavior and motor activity. The results showed that mice treated with the phenotyping pipeline had significantly lower grip strength than naïve and ECG-treated mice. Spruce bedding was associated with increased time spent in the center area in the open field test. In addition, in the rotarod test, the falling latency on corncob bedding was significantly shorter than on birch bedding. Furthermore, TMC has observed strain-specific phenotypic differences in drug responses. For instance, the open field test demonstrated significantly higher activity in C57BL/6J mice, and telemetry ECGs indicated the presence of T waves in the CD-1 and C57BL/6J strains but not in the C57BL/6N strain.
[Consideration]
TMC operates under a contract research organization (CRO) business model
[Conclusion]
TMC accepts SPF mice without quarantine procedures and utilizes mouse phenotyping to investigate gene functions, disease mechanisms, and translational medicine, operating under a CRO business model to advance preclinical research and drug candidate selection.
The Taiwan Mouse Clinic (TMC) is a specific pathogen-free (SPF) animal facility designed for comprehensive mouse phenotyping and drug testing, as well as specialized, customized services in disease model studies and exploratory safety pharmacology.
[Method]
To facilitate user-friendly services, TMC has developed a unique and straightforward Standard Operating Procedure (SOP) for the easy check-in of mice. This SOP allows researchers' SPF mice to be admitted directly into the facility without a quarantine period. In addition, mice are housed in separate, individually ventilated cages (IVC) carts or rooms, based on user groups, to ensure proper health monitoring and care. This practice has helped TMC maintain an 18-year record free of 12 specific pathogens. Notably, TMC has developed a highly effective method for “cleaning” mice that circumvents the need for re-derivation through embryo transfer or cesarean hysterectomy. In-depth studies have shown that environmental effects and assay interactions would affect mouse phenotyping.
[Results]
We found that assay interactions and environmental factors significantly influence mouse phenotyping, particularly the effects of phenotyping pipelines and bedding type on behavior and motor activity. The results showed that mice treated with the phenotyping pipeline had significantly lower grip strength than naïve and ECG-treated mice. Spruce bedding was associated with increased time spent in the center area in the open field test. In addition, in the rotarod test, the falling latency on corncob bedding was significantly shorter than on birch bedding. Furthermore, TMC has observed strain-specific phenotypic differences in drug responses. For instance, the open field test demonstrated significantly higher activity in C57BL/6J mice, and telemetry ECGs indicated the presence of T waves in the CD-1 and C57BL/6J strains but not in the C57BL/6N strain.
[Consideration]
TMC operates under a contract research organization (CRO) business model
[Conclusion]
TMC accepts SPF mice without quarantine procedures and utilizes mouse phenotyping to investigate gene functions, disease mechanisms, and translational medicine, operating under a CRO business model to advance preclinical research and drug candidate selection.
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