[P008] Poster

1:00 PM - 1:50 PM JST(4:00 AM - 4:50 AM UTC)

[P04-490] Antioxidant Potential of Single and Combined Extracts of Chrysanthemum and Cassia Seed in HaCaT Cells

○Xin-Ling Luo¹, Yu-Hsiang Xiao¹, Yi-Ping Yu¹ (1. Chinese Culture University, Taipei, Taiwan (Taiwan))

Keywords:

Chrysanthemum morifolium Ram,Cassia tora Linn,combined extract,antioxidant activity,oxidative stress

Chrysanthemum morifolium Ram., a perennial herb of the Asteraceae family, is rich in vitamins, minerals, and flavonoid antioxidants and has been reported to possess anti-aging and immunomodulatory properties. Cassia toraLinn., a member of the Leguminosae family, contains abundant bioactive compounds, including polyphenols and flavonoids, and exhibits strong antioxidant activity with potential skin-related applications. In this study, aqueous extracts of C. morifolium, C. tora, and their combination (2:1) were prepared by boiling for 30 min. Human immortalized keratinocytes (HaCaT cells) were used as an exo vivo model to evaluate the effects of these extracts on cell viability under oxidative stress conditions. In the cytotoxicity assay, none of the treatments exhibited cytotoxicity, and cell viability remained above 100% in all groups. In the H₂O₂-induced oxidative stress model, the control group showed a cell viability of approximately 43%, whereas all treatment groups significantly increased cell viability compared with the control group (p < 0.05). Among them, C. tora showed the strongest protective effect at 0.25 mg/mL. In the light-induced oxidative stress assay, blue light was used as the stress-inducing control, while red light served as the blank control representing normal light conditions. Blue light irradiation reduced cell viability to approximately 48%, indicating effective induction of oxidative damage. Under blue light-induced stress, all extracts attenuated oxidative damage and improved cell viability. These findings indicate that both C. morifolium and C. tora extracts possess antioxidant and cytoprotective properties. The combined extract promoted cell activity, whereas C. tora alone exhibited the best protection against H₂O₂-induced oxidative stress, suggesting that it may be the major contributor to the observed antioxidant activity. However, no consistently significant protective effect was observed under light-induced oxidative stress conditions, indicating that the effects may vary depending on the source of oxidative stress and warrant further investigation.