Presentation Information
[P04-572]TGF-β-Guided Discovery of Upcycled Salmon Head Peptides for Skin Regeneration and UV Protection
○Yun-Su Lee1, Wook-Chul Kim1, Seo-Rin Jung1, Ye-Sol Kim1, Seung-Hong Lee1 (1. Department of Medical Science, Soonchunhyang University, Asan, Republic of Korea (Korea))
Keywords:
Salmon head hydrolysates,Protamex-derived peptides,TGF-β induction,Skin regeneration,UV protection
[Purpose]
To upcycle and valorize salmon head, a major fishery byproduct, this study aimed to generate peptide-rich hydrolysates using Protamex and identify bioactive peptides with potential cosmeceutical applications for skin moisturization, regeneration, and UV protection through a TGF-β-guided screening process. [Method]
Salmon head hydrolysates were prepared by enzymatic digestion with Protamex and fractionated by tangential flow filtration into three molecular weight groups: <1 kDa, 1–3 kDa, and >3 kDa. Based on their ability to induce TGF-β expression in HaCaT keratinocytes and UVB-irradiated human dermal fibroblasts (HDFs), the <1 kDa fraction was selected for further purification. This fraction was separated by Sephadex chromatography into four subfractions (F1–F4), and F4, which showed the strongest activity, was further analyzed to isolate six peptides (1P–6P). These peptides were then comparatively evaluated for their biological activities in skin-related cell models.
[Results]
In HaCaT keratinocytes, peptide 5P showed the strongest induction of TGF-β expression and promoted the upregulation of tight junction factors. In UVB-irradiated HDFs, peptide 6P exhibited the highest TGF-β induction, which led to activation of the SMAD signaling pathway as well as enhanced cell proliferation and photoprotective responses. Overall, the selected peptides demonstrated significant skin-beneficial bioactivities associated with barrier function, regeneration, and UV defense.
[Consideration]
These findings suggest that low-molecular-weight peptides derived from salmon head hydrolysates can serve as effective functional ingredients for skin health. The study is particularly meaningful in that it presents a sustainable strategy for converting fishery byproducts into high-value bioactive compounds. In addition, the TGF-β-guided selection approach proved useful for identifying peptides with distinct functional effects in different skin cell types. [Conclusion]
Protamex-derived peptides from salmon head hydrolysates, especially peptides 5P and 6P selected through a TGF-β-guided fractionation process, show strong potential as upcycled marine bioactive ingredients for cosmeceutical applications targeting skin moisturization, regeneration, and protection against UV-induced damage.
To upcycle and valorize salmon head, a major fishery byproduct, this study aimed to generate peptide-rich hydrolysates using Protamex and identify bioactive peptides with potential cosmeceutical applications for skin moisturization, regeneration, and UV protection through a TGF-β-guided screening process. [Method]
Salmon head hydrolysates were prepared by enzymatic digestion with Protamex and fractionated by tangential flow filtration into three molecular weight groups: <1 kDa, 1–3 kDa, and >3 kDa. Based on their ability to induce TGF-β expression in HaCaT keratinocytes and UVB-irradiated human dermal fibroblasts (HDFs), the <1 kDa fraction was selected for further purification. This fraction was separated by Sephadex chromatography into four subfractions (F1–F4), and F4, which showed the strongest activity, was further analyzed to isolate six peptides (1P–6P). These peptides were then comparatively evaluated for their biological activities in skin-related cell models.
[Results]
In HaCaT keratinocytes, peptide 5P showed the strongest induction of TGF-β expression and promoted the upregulation of tight junction factors. In UVB-irradiated HDFs, peptide 6P exhibited the highest TGF-β induction, which led to activation of the SMAD signaling pathway as well as enhanced cell proliferation and photoprotective responses. Overall, the selected peptides demonstrated significant skin-beneficial bioactivities associated with barrier function, regeneration, and UV defense.
[Consideration]
These findings suggest that low-molecular-weight peptides derived from salmon head hydrolysates can serve as effective functional ingredients for skin health. The study is particularly meaningful in that it presents a sustainable strategy for converting fishery byproducts into high-value bioactive compounds. In addition, the TGF-β-guided selection approach proved useful for identifying peptides with distinct functional effects in different skin cell types. [Conclusion]
Protamex-derived peptides from salmon head hydrolysates, especially peptides 5P and 6P selected through a TGF-β-guided fractionation process, show strong potential as upcycled marine bioactive ingredients for cosmeceutical applications targeting skin moisturization, regeneration, and protection against UV-induced damage.
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