Presentation Information
[P04-576]Marine Algal Exosomes: A Novel Approach to Hair Regeneration
○Seo-rin Jung1, Wook-chul Kim1, Yun-su Lee1, Ye-sol Kim1, Seung-hong Lee1 (1. Department of Medical Science, Soonchunhyang University, Asan, Republic of Korea (Korea))
Keywords:
Seaweed,alopecia,exosomes,human dermal papilla cells,hair growth
[Purpose]Interest in hair loss treatment has increased with the growing importance of personal appearance and quality of life. Exosomes have recently attracted attention as bioactive materials due to their role in intercellular communication and delivery of functional molecules. While most exosome research has focused on animal-derived sources, studies on plant and algal-derived exosomes are still limited despite their advantages in terms of safety, sustainability, and resource availability. Seaweed is a marine biomass rich in polysaccharides and antioxidant compounds and has been widely studied for applications in food, pharmaceuticals, and cosmetics. Therefore, this study aimed to isolate exosomes from seaweed using various extraction methods and evaluate their potential effects on hair growth.
[Method] To obtain seaweed-derived exosomes, multiple extraction processes were performed. Enzymatic extraction was conducted using four enzymes, Viscozyme, Celluclast, Termamyl, and AMG, and ultrasonic extraction was applied as an alternative extraction method. The extracts were subsequently filtered and concentrated using a 200 nm pore-size tangential flow filtration (TFF) system. Exosomes were further isolated using an exosome separation column. The isolated particles were characterized by nanoparticle tracking analysis (NTA) to determine particle size and concentration, and western blot analysis was performed to confirm the presence of exosome marker proteins. The isolated exosomes were then treated in human dermal papilla cells (HDPCs) to evaluate their effects on hair growth-related cellular responses.
[Results]NTA results confirmed that particles within the exosome size range were successfully isolated, and western blot analysis verified the presence of major exosome marker proteins. When seaweed-derived exosomes were treated in HDPCs, cell proliferation increased compared to the control group. In addition, the expression of hair growth-related protein factors was upregulated, indicating that the exosomes had a positive effect on dermal papilla cell activity related to hair growth. [Consideration]These results suggest that seaweed-derived exosomes contain bioactive molecules that can influence dermal papilla cell behavior and may contribute to hair growth-related mechanisms. Compared to conventional extract-based materials, exosomes may provide advantages such as improved delivery efficiency and stability of bioactive compounds. Furthermore, the use of marine biomass such as seaweed provides a sustainable and scalable resource for bioactive material development. [Conclusion]In conclusion, seaweed-derived exosomes were successfully isolated using enzymatic and ultrasonic extraction methods combined with TFF and column separation. The isolated exosomes promoted proliferation of human dermal papilla cells and increased the expression of hair growth-related proteins. These findings indicate that seaweed-derived exosomes have potential as novel bioactive materials for hair growth applications in cosmetic and biomedical fields.
[Method] To obtain seaweed-derived exosomes, multiple extraction processes were performed. Enzymatic extraction was conducted using four enzymes, Viscozyme, Celluclast, Termamyl, and AMG, and ultrasonic extraction was applied as an alternative extraction method. The extracts were subsequently filtered and concentrated using a 200 nm pore-size tangential flow filtration (TFF) system. Exosomes were further isolated using an exosome separation column. The isolated particles were characterized by nanoparticle tracking analysis (NTA) to determine particle size and concentration, and western blot analysis was performed to confirm the presence of exosome marker proteins. The isolated exosomes were then treated in human dermal papilla cells (HDPCs) to evaluate their effects on hair growth-related cellular responses.
[Results]NTA results confirmed that particles within the exosome size range were successfully isolated, and western blot analysis verified the presence of major exosome marker proteins. When seaweed-derived exosomes were treated in HDPCs, cell proliferation increased compared to the control group. In addition, the expression of hair growth-related protein factors was upregulated, indicating that the exosomes had a positive effect on dermal papilla cell activity related to hair growth. [Consideration]These results suggest that seaweed-derived exosomes contain bioactive molecules that can influence dermal papilla cell behavior and may contribute to hair growth-related mechanisms. Compared to conventional extract-based materials, exosomes may provide advantages such as improved delivery efficiency and stability of bioactive compounds. Furthermore, the use of marine biomass such as seaweed provides a sustainable and scalable resource for bioactive material development. [Conclusion]In conclusion, seaweed-derived exosomes were successfully isolated using enzymatic and ultrasonic extraction methods combined with TFF and column separation. The isolated exosomes promoted proliferation of human dermal papilla cells and increased the expression of hair growth-related proteins. These findings indicate that seaweed-derived exosomes have potential as novel bioactive materials for hair growth applications in cosmetic and biomedical fields.
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