Presentation Information
[P01-902]Verification of Tryptophan-free aaRS Activity for the Development of an Artificial Translation System Composed of 19 Amino Acids without Tryptophan
〇Ayano Hatako1, Daisuke Kiga1 (1. School of Advanced Science and Engineering, Waseda University)
19 amino acids without tryptophan.
The purpose of this study was to develop aminoacyl-transfer RNA synthetases (aaRS) that function without tryptophan residue (Trp-free aaRS) for creating an artificial translation system composed of 19 amino acids except for tryptophan. We have established a simplified genetic code in which Trp is not translated using a cell-free protein synthesis system (Kawahara-Kobayashi et al., 2012). Hagino et al. have succeeded in establishing a sustainable protein synthesis system using aaRS produced within the in vitro protein synthesis system (Hagino et al., 2025). The development of Trp-free aaRS is expected to lead to the creation of a sustainable Trp-free protein synthesis system. Constructing artificial protein synthesis system, and even artificial life, that functions using the 19 amino acids other than tryptophan will help us understand how ancient organisms functioned without tryptophan.
While there are several methods for building Trp-free aaRS, in this study, we first employed a method that utilizes wild-type sequences. For some kinds of aaRSs, sequences that do not contain Trp are present in UniProt, a protein database. Here, we report the latest findings on Trp-free aaRS, for which we evaluated its activity.
The purpose of this study was to develop aminoacyl-transfer RNA synthetases (aaRS) that function without tryptophan residue (Trp-free aaRS) for creating an artificial translation system composed of 19 amino acids except for tryptophan. We have established a simplified genetic code in which Trp is not translated using a cell-free protein synthesis system (Kawahara-Kobayashi et al., 2012). Hagino et al. have succeeded in establishing a sustainable protein synthesis system using aaRS produced within the in vitro protein synthesis system (Hagino et al., 2025). The development of Trp-free aaRS is expected to lead to the creation of a sustainable Trp-free protein synthesis system. Constructing artificial protein synthesis system, and even artificial life, that functions using the 19 amino acids other than tryptophan will help us understand how ancient organisms functioned without tryptophan.
While there are several methods for building Trp-free aaRS, in this study, we first employed a method that utilizes wild-type sequences. For some kinds of aaRSs, sequences that do not contain Trp are present in UniProt, a protein database. Here, we report the latest findings on Trp-free aaRS, for which we evaluated its activity.
Comment
To browse or post comments, you must log in.Log in