[P002] Poster

1:00 PM - 1:50 PM JST(4:00 AM - 4:50 AM UTC)

[P01-906] Advancing cyanobacteria as the next-generation biofactory

¹ Carbon-Negative Synthetic Biology for Biomaterial Production from CO2 (CNSB), Campus for Research Excellence and Technological Enterprise (CREATE), Singapore.
² NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), National University of Singapore.
³ Synthetic Biology Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore.
⁴ Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore.
⁵ National Centre for Engineering Biology (NCEB), Singapore.

Keywords:

Picosynechococcus sp. PCC 7002,PCC 11901,2,3-butanediol (2,3-BDO),next-generation biofactory,directed-accelerated evolution.

Biomanufacturing, which deploys cyanobacteria as novel production chassis, has been considered a potential approach to tackle global warming issues and achieve a circular economy. Picosynechococcus sp. PCC 7002 and PCC 11901, two marine cyanobacteria, possess key characteristics of an industrial host, including their fast growth rate and high tolerance to severe environmental conditions such as high salt, light, and temperature. We aimed to demonstrate their potential as the next-generation biofactory by engineering them for the sustainable production of the biofuel 2,3-butanediol (2,3-BDO). Through screening various genetic components, we identified two gene combinations (budB-budA and alsS-alsD) for transformation into PCC 7002 and PCC 11901. Subsequently, several candidate genes for 2,3-BDO dehydrogenases are screened in combination with the 2-gene clusters to obtain the final recombinant strain with the highest 2,3-BDO titer. Simultaneously, an EvolvR-based system is developed for the directed-accelerated evolution in the recombinant strains targeting the 2,3-BDO production gene components to achieve robust production in the final recombinants. Furthermore, this system also facilitates targeted in vivo mutagenesis to improve growth rates and carbon fixation efficiency, broadening the applicability of cyanobacteria as robust microbial cell factories.