Presentation Information
[IO-6]Rapid polymerizing pH-responsive multifunctional hydrogel for periodontal disease treatment and periodontal bone regeneration
*Rui Mu1, Yingnan Tian2, shijia Huang1, Minxin He1, Lei Jin1,2 (1. Department of Orthodontics & Prosthodontics, The Second Affiliated Hospital of Zhejiang University School of Medicine, 2. Department of Stomatology, The First Affiliated Hospital of Nanjing Medical University)
[Objective]
Periodontal disease is a chronic inflammatory condition requiring precise treatment. To address limitations of existing drug delivery systems, we developed a pH-responsive anti-inflammatory hydrogel (CPPI) for periodontitis. This hydrogel degrades in acidic, inflamed conditions, enabling targeted drug release via ultraviolet polymerization, enhancing therapeutic efficacy.
[Method]
The CPPI was synthesized by modifying carboxymethyl chitosan with phenylboronic acid and incorporating IGF-1-loaded polydopamine via UV irradiation. Its properties were analyzed using FTIR and rheological tests. In vitro (CCK-8, DPPH, qRT-PCR, Western Blot, antibacterial assays) and in vivo studies confirmed its anti-inflammatory and osteogenic effects. Transcriptome sequencing explored the mechanisms. The study was approved by the Laboratory Animal Ethics Committee of Zhejiang University School (approval number: AIRB-2024-2107).
[Results and Discussion]
FTIR and rheological tests confirmed CPPI’s properties. Drug release increased in inflammatory conditions. In vitro, CPPI reduced ROS, IL-6, TNF-α, and increased OCN and RUNX2, showing anti-inflammatory, osteogenic, and bactericidal effects. In vivo, it reduced periodontal inflammation and bone resorption, potentially via cell cycle pathways, indicating its potential for periodontitis treatment.
[References]
1) XY Wang, HJ Zhao, ZC Liu, et al. Polydopamine nanoparticles as dual-task platform for osteoarthritis therapy: A scavenger for reactive oxygen species and regulator for cellular powerhouses. CHEM ENG J 2021; 417: 129284. https://doi.org/10.1016/j.cej.2021.129284
2) JX Yang, WN Zeng, P Xu, et al. Glucose-responsive multifunctional metal-organic drug-loaded hydrogel for diabetic wound healing. Acta Biomater 2022; 140: 206-18. https://doi.org/10.1016/j.actbio.2021.11.043
3) S Xu, B Hu, T Dong, et al. Alleviate Periodontitis and Its Comorbidity Hypertension using a Nanoparticle-Embedded Functional Hydrogel System. Adv Healthc Mater 2023; 12(20): e2203337. https://doi.org/10.1002/adhm.202203337
4) Y Liu, T Li, ML Sun, et al. ZIF-8 modified multifunctional injectable photopolymerizable GelMA hydrogel for the treatment of periodontitis. Acta Biomater 2022; 146: 37-48. https://doi.org/10.1016/j.actbio.2022.03.046
5) GS Han, DW Domaille. Connecting the dynamics and reactivity of arylboronic acids to emergent and stimuli-responsive material properties. J Mater Chem B 2022; 10(33): 6263- https://doi.org/10.1039/d2tb00968d
Periodontal disease is a chronic inflammatory condition requiring precise treatment. To address limitations of existing drug delivery systems, we developed a pH-responsive anti-inflammatory hydrogel (CPPI) for periodontitis. This hydrogel degrades in acidic, inflamed conditions, enabling targeted drug release via ultraviolet polymerization, enhancing therapeutic efficacy.
[Method]
The CPPI was synthesized by modifying carboxymethyl chitosan with phenylboronic acid and incorporating IGF-1-loaded polydopamine via UV irradiation. Its properties were analyzed using FTIR and rheological tests. In vitro (CCK-8, DPPH, qRT-PCR, Western Blot, antibacterial assays) and in vivo studies confirmed its anti-inflammatory and osteogenic effects. Transcriptome sequencing explored the mechanisms. The study was approved by the Laboratory Animal Ethics Committee of Zhejiang University School (approval number: AIRB-2024-2107).
[Results and Discussion]
FTIR and rheological tests confirmed CPPI’s properties. Drug release increased in inflammatory conditions. In vitro, CPPI reduced ROS, IL-6, TNF-α, and increased OCN and RUNX2, showing anti-inflammatory, osteogenic, and bactericidal effects. In vivo, it reduced periodontal inflammation and bone resorption, potentially via cell cycle pathways, indicating its potential for periodontitis treatment.
[References]
1) XY Wang, HJ Zhao, ZC Liu, et al. Polydopamine nanoparticles as dual-task platform for osteoarthritis therapy: A scavenger for reactive oxygen species and regulator for cellular powerhouses. CHEM ENG J 2021; 417: 129284. https://doi.org/10.1016/j.cej.2021.129284
2) JX Yang, WN Zeng, P Xu, et al. Glucose-responsive multifunctional metal-organic drug-loaded hydrogel for diabetic wound healing. Acta Biomater 2022; 140: 206-18. https://doi.org/10.1016/j.actbio.2021.11.043
3) S Xu, B Hu, T Dong, et al. Alleviate Periodontitis and Its Comorbidity Hypertension using a Nanoparticle-Embedded Functional Hydrogel System. Adv Healthc Mater 2023; 12(20): e2203337. https://doi.org/10.1002/adhm.202203337
4) Y Liu, T Li, ML Sun, et al. ZIF-8 modified multifunctional injectable photopolymerizable GelMA hydrogel for the treatment of periodontitis. Acta Biomater 2022; 146: 37-48. https://doi.org/10.1016/j.actbio.2022.03.046
5) GS Han, DW Domaille. Connecting the dynamics and reactivity of arylboronic acids to emergent and stimuli-responsive material properties. J Mater Chem B 2022; 10(33): 6263- https://doi.org/10.1039/d2tb00968d
