Presentation Information
[IO2-2]Title: Interface Engineering of 1.5YSZ Implants Using ABL-(Zn/Cu/Sr)-Tannic Acid Metal-Phenolic Networks
Sub Title: An In Vitro Study of Bioactivity and Osteogenesis
*Zhiwei Zhou1, Kosuke Nozaki1, Satsuki Tanaka1, Hao Wu1, Xiaohe Zhou1, Noriyuki Wakabayashi1 (1. Advanced Prosthodontics, Division of Oral Health Sciences, Graduate School, Institute of Science Tokyo)
[Objective]
1.5 mol% yttria-stabilized zirconia (1.5YSZ) possesses superior mechanical properties for dental implants but lacks bioactivity. Recent advances in interface engineering suggest that metal–phenolic networks (MPNs) provide a versatile platform for functional surface modification through supramolecular coordination chemistry.This study engineered multifunctional nano-coatings on 1.5YSZ using metal-phenolic networks (MPNs) incorporating tannic acid (TA), abaloparatide (ABL), and metal ions (Zn, Cu, Sr) [1]. Specifically, this study aimed to (i) construct stable ABL-loaded MPN coatings on 1.5YSZ surfaces, (ii) elucidate the effects of different metal ion combinations on surface physicochemical properties, and (iii) evaluate their influence on osteoblast viability, differentiation, and mineralization in vitro, with the goal of enhancing zirconia–bone integration.
[Method]
1.5YSZ discs were coated with TA-ABL complexes coordinated with metal ions, forming ABL@ZnTA (Zn), ABL@ZnCuTA (ZnCu), and ABL@ZnSrTA (ZnSr) groups. Surface characteristics were analyzed via scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), and Fourier transform infrared spectroscopy (FTIR). Osteoblast-like MC3T3-E1 cells were seeded to evaluate cytotoxicity (CCK-8), osteogenic differentiation (ALP activity), and mineralization (Alizarin Red S staining) over 28 days.
[Results and Discussion]
SEM and FTIR confirmed successful coating assembly, which improved hydrophilicity without cytotoxicity. While all coated groups enhanced osteogenesis compared to uncoated 1.5YSZ, the ZnSr group exhibited superior performance. The Zn group showed an early ALP peak followed by a rapid decline, whereas the ZnSr group maintained high ALP activity through Day 14, suggesting a prolonged window of osteogenic stimulation [2]. Consequently, ZnSr induced the most robust mineralization at Day 28. These findings indicate that ABL@ZnSrTA synergizes zirconia's stability with the bioactivity of ABL and strontium, offering a promising strategy for advanced implants [3].
[References]
1.Xu L, Fang J, Pan J, et al. Zinc finger-inspired peptide-metal-phenolic nanointerface enhances bone-implant integration. Bioact Mater 2024; 41.
2.Aimaiti A, Mai A, Boyong X, et al. Low-dose strontium stimulates osteogenesis but high-dose doses cause apoptosis. Stem Cell Res Ther 2017; 8.
3.Zhong Z, Wu X, Wang Y, et al. Zn/Sr dual ions-collagen co-assembly hydroxyapatite enhances bone regeneration. Bioact Mater 2022; 10.
1.5 mol% yttria-stabilized zirconia (1.5YSZ) possesses superior mechanical properties for dental implants but lacks bioactivity. Recent advances in interface engineering suggest that metal–phenolic networks (MPNs) provide a versatile platform for functional surface modification through supramolecular coordination chemistry.This study engineered multifunctional nano-coatings on 1.5YSZ using metal-phenolic networks (MPNs) incorporating tannic acid (TA), abaloparatide (ABL), and metal ions (Zn, Cu, Sr) [1]. Specifically, this study aimed to (i) construct stable ABL-loaded MPN coatings on 1.5YSZ surfaces, (ii) elucidate the effects of different metal ion combinations on surface physicochemical properties, and (iii) evaluate their influence on osteoblast viability, differentiation, and mineralization in vitro, with the goal of enhancing zirconia–bone integration.
[Method]
1.5YSZ discs were coated with TA-ABL complexes coordinated with metal ions, forming ABL@ZnTA (Zn), ABL@ZnCuTA (ZnCu), and ABL@ZnSrTA (ZnSr) groups. Surface characteristics were analyzed via scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), and Fourier transform infrared spectroscopy (FTIR). Osteoblast-like MC3T3-E1 cells were seeded to evaluate cytotoxicity (CCK-8), osteogenic differentiation (ALP activity), and mineralization (Alizarin Red S staining) over 28 days.
[Results and Discussion]
SEM and FTIR confirmed successful coating assembly, which improved hydrophilicity without cytotoxicity. While all coated groups enhanced osteogenesis compared to uncoated 1.5YSZ, the ZnSr group exhibited superior performance. The Zn group showed an early ALP peak followed by a rapid decline, whereas the ZnSr group maintained high ALP activity through Day 14, suggesting a prolonged window of osteogenic stimulation [2]. Consequently, ZnSr induced the most robust mineralization at Day 28. These findings indicate that ABL@ZnSrTA synergizes zirconia's stability with the bioactivity of ABL and strontium, offering a promising strategy for advanced implants [3].
[References]
1.Xu L, Fang J, Pan J, et al. Zinc finger-inspired peptide-metal-phenolic nanointerface enhances bone-implant integration. Bioact Mater 2024; 41.
2.Aimaiti A, Mai A, Boyong X, et al. Low-dose strontium stimulates osteogenesis but high-dose doses cause apoptosis. Stem Cell Res Ther 2017; 8.
3.Zhong Z, Wu X, Wang Y, et al. Zn/Sr dual ions-collagen co-assembly hydroxyapatite enhances bone regeneration. Bioact Mater 2022; 10.