Presentation Information
[IO2-3]In vitro evaluation of biocompatibility and osteoconductive potential of ultrashort pulsed laser–treated ceria-stabilized zirconia/alumina nanocomposite surfaces as material for dental implants.
*Bryan Martin del Campo1, Minoru Sanda1, Shoma Yamamori1, Takahito Osawa1, Yoko Oshima1, Eri Urano-Morisawa1, Ayako Mochizuki2, Fuminori Iwasa3, Kazuyoshi Baba1 (1. Department of Prosthodontics, Showa Medical University, School of Dentistry, 2. Department of Oral Physiology, Showa Medical University, School of Dentistry, 3. Division of Fixed Prosthodontics, Department of Restorative and Biomaterials Sciences, Meikai University, School of Dentistry)
[Objective]
This study evaluated the surface characteristics and osteoconductive potential of ultrashort pulsed laser–treated ceria–stabilized zirconia/alumina (Ce-TZP/Al2O3) nanocomposite in vitro.
[Method]
Disk-shaped specimens (20.0 mm diameter, 1.0 mm thickness) made of Ce-TZP/Al2O3 were prepared. Untreated specimens with a machined surface served as controls, whereas the experimental specimens were subjected to ultrashort-pulsed laser surface texturing. The surface topography and chemical states were analyzed using scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS). Osteoconductive potential was evaluated using MC3T3-E1 pre-osteoblastic cells by measuring cell adhesion (3 and 24 hours), proliferation (5 days), and alkaline phosphatase (ALP) activity on days 7, 14, and 21 (n = 4). Osteogenic gene expression of type I collagen (Col1a1), osterix (Sp7), osteopontin (Spp1), and osteocalcin (Bglap) was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) at 3, 7, and 14 days (n = 3).
[Results and Discussion]
Control specimens exhibited smooth and flat surfaces, while laser-treated specimens showed micro- and nanostructured topography with greater surface area, roughness, and pronounced undercuts (1). In vitro assays demonstrated a significant increase in cell adhesion, proliferation, and ALP activity in laser-treated Ce-TZP/Al2O3nanocomposites compared with controls, and the qRT-PCR analysis indicated higher expression of osteogenic-related genes, with upregulation of Sp7 at day 3, Spp1 at days 3 and 7, and Bglap at days 7 and 14 (2).
Ultrashort pulsed laser–induced macro- and nanostructuring of Ce-TZP/Al2O3 surfaces significantly improved osteoconductive responses in vitro, suggesting its potential as a dental implant material.
[References]
1) Li Y, et al. Enhanced osteoconductivity of zirconia implants with one-step femtosecond laser treatment through morphological and chemical modifications. J Funct Biomater 2025; 16(4): 142.
2) Oshima Y, et al. Effect of nanofeatured topography on ceria-stabilized zirconia/alumina nanocomposite on osteogenesis and osseointegration. Int J Oral Maxillofac Implants 2017; 32(1): 81–91.
This study evaluated the surface characteristics and osteoconductive potential of ultrashort pulsed laser–treated ceria–stabilized zirconia/alumina (Ce-TZP/Al2O3) nanocomposite in vitro.
[Method]
Disk-shaped specimens (20.0 mm diameter, 1.0 mm thickness) made of Ce-TZP/Al2O3 were prepared. Untreated specimens with a machined surface served as controls, whereas the experimental specimens were subjected to ultrashort-pulsed laser surface texturing. The surface topography and chemical states were analyzed using scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS). Osteoconductive potential was evaluated using MC3T3-E1 pre-osteoblastic cells by measuring cell adhesion (3 and 24 hours), proliferation (5 days), and alkaline phosphatase (ALP) activity on days 7, 14, and 21 (n = 4). Osteogenic gene expression of type I collagen (Col1a1), osterix (Sp7), osteopontin (Spp1), and osteocalcin (Bglap) was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) at 3, 7, and 14 days (n = 3).
[Results and Discussion]
Control specimens exhibited smooth and flat surfaces, while laser-treated specimens showed micro- and nanostructured topography with greater surface area, roughness, and pronounced undercuts (1). In vitro assays demonstrated a significant increase in cell adhesion, proliferation, and ALP activity in laser-treated Ce-TZP/Al2O3nanocomposites compared with controls, and the qRT-PCR analysis indicated higher expression of osteogenic-related genes, with upregulation of Sp7 at day 3, Spp1 at days 3 and 7, and Bglap at days 7 and 14 (2).
Ultrashort pulsed laser–induced macro- and nanostructuring of Ce-TZP/Al2O3 surfaces significantly improved osteoconductive responses in vitro, suggesting its potential as a dental implant material.
[References]
1) Li Y, et al. Enhanced osteoconductivity of zirconia implants with one-step femtosecond laser treatment through morphological and chemical modifications. J Funct Biomater 2025; 16(4): 142.
2) Oshima Y, et al. Effect of nanofeatured topography on ceria-stabilized zirconia/alumina nanocomposite on osteogenesis and osseointegration. Int J Oral Maxillofac Implants 2017; 32(1): 81–91.