[17a-A31-1~5] 4.3 Laser sources and Laser applications

11:15 AM - 11:30 AM JST(2:15 AM - 2:30 AM UTC)English Presentation

[17a-A31-3] Utilizing Near-Infrared Femtosecond Laser-Generated Gas Bubbles for Acellular Area Construction in Cell Monolayers

〇Kazunori Okano^1,4, Naomi Tanga^1,2, Rieko Aida⁴, Hayato Suwa⁴, Hiromi Hagiwara⁴, Yalikun Yaxiaer¹, Koichiro Kishima⁵, Yoichiroh Hosokawa^1,2,3 (1.Mat. Sci, NAIST, 2.CDG, NAIST, 3.MediLux, NAIST, 4.Toin Yokohama Univ., 5.Pinpoint Photonics)

Keywords:

femtosecond laser,gas bubble,cell motility

The focused irradiation of near-infrared femtosecond laser (NIR fs laser) pulses in biological solvents efficiently induces molecular breakdown, generating vaporous gas micro bubbles. Such irradiation on live cells induces apoptosis and necrosis depending in pulse energy. Here we examined the relation bubble size and numbers and pulse energy in cells. Further, the NIR fs laser bubbling-formed cell-removed acellular area was used for assaying antimotility effects of the plant flavonoid apigenin.