Presentation Information
[18a-P01-8]Optical Trapping Induced Surface Deformation At Protein Solution
〇(M1)Chia-Hong Su1, Po-Wei Yi1, Chih-Hao Huang1, Mu-En Li1, Hiroshi Masuhara1 (1.Nat'l Yang Ming Chiao Tung Univ.,Taiwan)
Keywords:
Cooperative optical trapping,Protein assembly formation,Surface deformation
Optical trapping is an innovative technique for controlling small substances inside the laser focus, such as protein molecules. In our group, we adopted polystyrene microparticles (PS MPs) to help us figure out the protein assembly during the lasing action. Upon turning on the trapping laser, protein clusters are trapped, forming the assembly larger than the laser focus. Surprisingly, we found a linear stick-like assembly of PS MPs following the growth of the protein assembly edge, and its surface lift-up just by pending the solution for 1 hour before switching on the trapping laser. The observation suggests that optical force is stronger than surface tension, leading to this interesting breakthrough behavior of solution surface.
In figure 1(a), all PS MPs of 6 μm diameter after pending 1 hour are well dispersed on the solution surface due to the buoyancy. As switching on the trapping laser, optical force strongly exerts pressure on the protein assembly resulting in surface deformation. The transmission intensity of PS MPs around the focus gives large difference, indicating that the height in z-direction of PS MPs near the laser focus is higher than the original state. Solution without lysozyme protein is also done as a control group, showed in the figure 1(b). PS MPs formed the hexagonal packing, which is consistent with our previous research. We also determine the height change of surface deformation, which is around 3.65 μm by making and using correlation curves, given in Figure 2. This new finding phenomenon offers a viewpoint of understanding optical trapping at the air solution surface.
In figure 1(a), all PS MPs of 6 μm diameter after pending 1 hour are well dispersed on the solution surface due to the buoyancy. As switching on the trapping laser, optical force strongly exerts pressure on the protein assembly resulting in surface deformation. The transmission intensity of PS MPs around the focus gives large difference, indicating that the height in z-direction of PS MPs near the laser focus is higher than the original state. Solution without lysozyme protein is also done as a control group, showed in the figure 1(b). PS MPs formed the hexagonal packing, which is consistent with our previous research. We also determine the height change of surface deformation, which is around 3.65 μm by making and using correlation curves, given in Figure 2. This new finding phenomenon offers a viewpoint of understanding optical trapping at the air solution surface.
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