[19p-C32-1~12] 3.3 Biomedical optics

3:30 PM - 3:45 PM JST(6:30 AM - 6:45 AM UTC)English Presentation

[19p-C32-10] Longitudinal time-lapse imaging of tumor spheroid’s drug response by an integrated
system of dynamic optical coherence tomography and cell cultivation

〇(P)Ibrahim Gamal Abd El-Sadek^1,2, Rion Morishita¹, Guo Yu¹, Atsuko Furukawa³, Shuichi Makita¹, Satoshi Matsusaka³, Yoshiaki Yasuno¹ (1.COG Univ. Tsukuba, 2.Damietta University, 3.Univ. of Tsukuba Med)

Keywords:

Dynamic OCT,Tumor spheroid,Drug testing

We present longitudinal time-lapse drug-response imaging of tumor spheroids using a combined cell cultivation chamber and dynamic optical coherence tomography (DOCT) microscope. The OCT-integrated cultivation chamber supplies the spheroid with 5 % CO₂ and keep its temperature at 37 ℃. This integrated system enables imaging a single sample with an arbitrary number of time points, and hence it enabled spheroid-drug-response assessment with high-time-resolution and small number of samples. The longitudinal DOCT patterns of non-treated and doxorubicin (DOX-) treated human breast cancer (MCF-7) spheroids have been successfully obtained over 100 hours.