[20a-P03-1~29] 12.6 Nanobiotechnology

[20a-P03-4] Quantitative Analysis of Extracellular Vesicles with Plasmonic Chips Immobilized with Different Capture Antibodies

〇(M1)Tatsuki Iwamura¹, Makoto Tokami¹, Yasunori Nawa¹, Keiko Tawa¹ (1.Kwansei Gakuin Univ.)

Keywords:

plasmon,immunosensor,exosome

Exosomes (Extracellular vesicles :EV) secreted from cells include various proteins, and it is possible to predict the diseases that will afflict in the future. However, it is difficult to detect a single Ev with about 100 nm diameter with an optical macroscope. In our laboratory, a single Ev was detected by fluorescence microscopy using a plasmonic chip with wavelength size periodic structures covered with a metallic film. In this study, membrane proteins included in Evs were quantitatively analyzed with a plasmonic chip immobilized with two-kinds of capture antibodies.