Presentation Information
[10a-N104-5]Biospeckle optical coherence tomography (bOCT) visualizing the impact of silver nanoparticle size on internal activity of lentil seeds
〇(D)Lavista Tyagi1, Hirofumi Kadono1, Uma Maheswari Rajagopalan2 (1.Graduate School of Science and Engineering, Saitama University, Japan, 2.Innovative Global Program, Shibaura Institute of Technology, Japan)
Keywords:
Biospeckle Optical Coherence Tomography (bOCT),Nanoparticles,Silver (Ag)
The rapid integration of nanoparticles (NPs) into various sectors, including agriculture, healthcare, and manufacturing, has raised concerns about their interactions with living organisms, particularly plants. Silver (Ag) NPs, renowned for their antimicrobial properties and widespread use in industry and biomedicine, are of particular interest because they can penetrate seed coats and affect cellular mechanisms during germination, underscoring their size-related impacts. This study utilizes Biospeckle Optical Coherence Tomography (bOCT), a non-contact, non-destructive, in vivo technique that visualizes and quantifies changes in internal physiological activity, to monitor lentil (Lens culinaris) seeds exposed to Ag NPs of various sizes during germination. Our earlier study effectively confirmed its ability to assess the size-dependent effects of CuO and Cu metal NPs on lentil seeds, indicating a substantial negative impact of smaller CuO and Cu NPs (<50 nm, 25 nm) at 100 mg/L, while larger particles (10 µm, 10-25 µm) showed positive effects.