Presentation Information
[8a-P07-1]Single Cell Dynamics on 1D Microgroove Substrate
〇Kazuhiro Aoki1, Hiromu Kuwabara1, Yuuta Moriyama1, Toshiyuki Mitsui1 (1.Aogaku Univ.)
Keywords:
fibroblasts,cardiomyocytes,biophysics
It is well known that cells respond to the surrounding geometric environment by migrating and orienting themselves accordingly, yet the processes underlying such behavior remain largely elusive. In this study, we co-cultured fibroblasts and cardiomyocytes derived from chick embryonic hearts on one-dimensional (1D) microgroove structures fabricated on PDMS substrates, and performed time-lapse imaging over a 48-hour period to observe how the cells migrated into and out of the grooves and established spatial order. Notably, we identified a subpopulation of cells that entered the grooves first, and found that cell motility dynamics depending on groove width.