Presentation Information
[10a-PB5-13]Development of a Single-Molecule Electrical Discrimination Method for Saccharide Isomers
〇(B)Jun Morita1, Takahito Ohshiro2, Yuki Komoto2, Masateru Taniguchi2 (1.Univ. of Osaka., 2.Univ. of Osaka)
Keywords:
Single-molecule measurement,Saccharide isomer,Tunnel current
Saccharides play important roles in biological processes, and their sensitive discrimination is important for glycan analysis and disease-maker detection. However, discrimination of monosaccharide isomers remains challenging because of their similar molecular structures and molecular weights. In this study, we investigated a single-molecule electrical discrimination method for saccharides isomers using mechanically controllable break junction (MCBJ) nanogap electrodes. Tunnel-current signals generated by individual monosaccharides passing through the nanogap were measured and analyzed. Characteristic features, including average current, peak current, dwell time, and current fluctuations, were extracted from the signals and classified using machine learning (XGBoost). As a result, glucose, galactose, and mannose were successfully discriminated with F-scores exceeding 80%. These results suggest that single-molecule tunnel-current measurements can electrically detect subtle stereochemical differences among saccharides.