[11a-C310-1~12] 3.1 Basic optics and frontier of optics

11:15 AM - 11:30 AM JST(2:15 AM - 2:30 AM UTC)Presentation by Applicant for JSAP Young Scientists Presentation Award

[11a-C310-9] Protein crystal nucleation from precipitant-free conditions via laser irradiation

〇Wataru Fushimoto¹, Ryo Suzuki², Tsuyoshi Inoue³, Masaru Tachibana², Hiroshi Yoshikawa¹ (1.UOsaka Eng., 2.Yokohama City Univ. Nanobio, 3.UOsaka Pharm. Sci.)

Keywords:

Laser trapping,Protein crystal,Crystallization

The use of lasers is gaining attention as a technique for crystallization control, especially in terms of spatiotemporal and polymorphic regulation. This study reports on crystallization under precipitant-free (salt-free) conditions, leveraging the local concentration effect induced by optical trapping via focused continuous-wave laser irradiation. Crystallization proceeded within 10 minutes at the focal spot, revealing that the crystallization pathways differ substantially between water and heavy-water conditions.