[8a-C214-1~13] 12.6 Nanobiotechnology

9:30 AM - 9:45 AM JST(12:30 AM - 12:45 AM UTC)

[8a-C214-3] Single-nucleotide sensing with a chemically stateful solid-state nanopore

〇Makusu Tsutsui¹, Yuki Komoto¹, Kazumichi Yokota², Wei-Lun Hsu³, Denis Garoli⁴, Ali Douaki⁴, German Lanzavecchia⁴, Ryuichiro Abe⁵, Hirofumi Daiguji³, Tomoji Kawai¹ (1.SANKEN, 2.AIST, 3.Univ. Tokyo, 4.IIT, 5.BIKEN)

Keywords:

nanopore,single molecule detection,machine learning

In this study, we used a solid-state nanopore that autonomously opens and closes through precipitation–dissolution reactions to detect single nucleotides passing through transient conductive pathways as ionic-current spike waveforms. Waveform analysis and machine learning enabled discrimination of the four nucleotides, demonstrating a single-molecule sensing platform that integrates a reaction field, memory, and sensing within one nanospace.