Presentation Information
[8a-PA2-24]Cell-level localization framework for alpha-emitting radionuclides using radioluminescence microscopy: Simulation study
〇(DC)Seohan Kim1,2,3, Jieun Han1,2,3, Wonmo Sung1,2,3 (1.Department of Biomedical Engineering, College of Medicine, The Catholic University of Korea, Seoul, South Korea, 2.Department of Medical Sciences, Graduate School of The Catholic University of Korea, Seoul, South Korea, 3.CMC Institute for Basic Medical Science, the Catholic Medical Center of The Catholic University of Korea, Seoul, South Korea)
Keywords:
Radioluminescence microscopy,Alpha-particle,Monte Carlo
Targeted alpha therapy (TAT) with 225Ac is nearing clinical use, but subcellular distributions of its decay daughters remain unmeasured. Radioluminescence microscopy (RLM) localizes alpha decays from scintillation tracks, but earlier ray-tracing models ignored diffraction blur and camera noise.
TOPAS framework produced 1000 single-alpha frames per energy at 4–8 MeV in a GAGG scintillator. Images at two focal planes near the scintillator entrance surface were generated using depth-dependent Gibson–Lanni PSF convolution and subsequently processed with an Andor iXon Ultra 888 EMCCD camera model. Sources were clustered by thresholding, DBSCAN, cross-plane matching, and ray extrapolation, with the noise filter tuned at 4 MeV for 70% detection.
Detection rate was 71–92% and median lateral error 6.5–7.7 μm across 4–8 MeV, with localization-PSF FWHM 13–16 μm. The framework yields cell-scale localization under realistic optical and EMCCD conditions, supporting RLM for cell-level dosimetry in TAT.
TOPAS framework produced 1000 single-alpha frames per energy at 4–8 MeV in a GAGG scintillator. Images at two focal planes near the scintillator entrance surface were generated using depth-dependent Gibson–Lanni PSF convolution and subsequently processed with an Andor iXon Ultra 888 EMCCD camera model. Sources were clustered by thresholding, DBSCAN, cross-plane matching, and ray extrapolation, with the noise filter tuned at 4 MeV for 70% detection.
Detection rate was 71–92% and median lateral error 6.5–7.7 μm across 4–8 MeV, with localization-PSF FWHM 13–16 μm. The framework yields cell-scale localization under realistic optical and EMCCD conditions, supporting RLM for cell-level dosimetry in TAT.