Presentation Information
[8p-C214-15]Asymmetric Lipid Fluidity in Microwell-Suspended Bilayers
〇Mana Honkawa1, Ayumu Tamura1, Azusa Oshima2, Aya Tanaka2, Akira Heya1, Koji Sumitomo1 (1.University of Hyogo, 2.NTT Basic Res. Labs/BMC)
Keywords:
membrane fluidity,lipid bilayer
In suspended membranes formed by spreading artificial bilayer membranes onto microwells fabricated on Si substrates using semiconductor microfabrication techniques, it has been reported that a diffusion barrier exists at the interface between the substrate-supported membrane and the suspended membrane in the case of SiO2/Si substrates. In this presentation, we report on the results of leaflet-specific membrane fluidity measurements using the fluorescence recovery after photobleaching (FRAP) method with asymmetric lipid bilayers, as well as the location of a diffusion barrier.