Presentation Information
[8p-E311-14]Enhancement of Chaetoceros gracilis Growth by Nanosecond-Pulsed Plasma-Irradiated Culture Medium
〇Takamasa Okumura1, Hiroaki Takebe2, Hibiki Otobe1, Atsushi Sakurai2, Sosuke Imamura2, Kazunori Koga1 (1.Kyushu Univ., 2.NTT)
Keywords:
plasma agriculture,Nanosecond pulsed plasma,plasma-treated culture medium
A nanosecond-pulsed plasma system for direct irradiation of liquid culture media was developed and applied to the cultivation of the marine diatom Chaetoceros gracilis. Uniform plasma emission was obtained from all electrodes while suppressing localized thermal discharges and temperature increases. Plasma-treated IMK medium enhanced cell growth during the late cultivation stage, and the optimal treatment condition increased cell density by approximately 15% compared with the untreated control. Metabolomic analysis using CE-FTMS revealed changes in metabolites related to membrane and pigment biosynthesis as well as nitrogen metabolism. These results suggest that nanosecond-pulsed plasma treatment improves nutrient utilization in the culture medium and contributes to sustained algal growth. The findings demonstrate the potential of direct plasma irradiation of liquid media as a novel strategy for promoting the cultivation of marine microalgae.