[9a-E301-1~11] 12.6 Nanobiotechnology

9:00 AM - 9:15 AM JST(12:00 AM - 12:15 AM UTC)Presentation by Applicant for JSAP Young Scientists Presentation Award

[9a-E301-1] Controlled fusion between lipid bilayer suspended over microwells and vesicles induced by pH gradient

〇Yurika Inoue¹, Kanta Tsumoto², Azusa Oshima³, Aya Tanaka³, Akira Heya¹, Koji Sumitomo¹ (1.Univ. of Hyogo, 2.Mie Univ., 3.NTT Basic Res. Labs/BMC)

Keywords:

Lipid bilayers suspended over microwells,Membrane fusion

We investigated the control of vesicle fusion with lipid bilayers suspended over microwells by regulating the pH of the surrounding solution. In an acidic solution, after dropping calcein-containing vesicles onto the suspended lipid bilayers containing negatively charged lipids and adding CaCl₂, calcein fluorescence was observed within the microwells. When the pH outside the vesicles was lower than that inside, proton permeation increased the proton concentration within the vesicles, causing the vesicles to become positively charged. As a result, electrostatic interactions occurred between the vesicles and the suspended lipid bilayers, and fusion was promoted by calcium ions.