[9p-PB1-1~22] 12.1 Fabrications and Structure Controls

[9p-PB1-18] DNA Translocation Dynamics near a Nanopore in PEG Aqueous Solutions
at Various Concentrations

〇(B)Yuto Shudo¹, Naoki Kato¹, Shuntaro Shimura¹, Yuuta Moriyama¹, Toshiyuki Mitsui¹ (1.Aogaku Univ.)

Keywords:

semiconductor,DNA,nanopore

DNA translocation and clogging events in polyethylene glycol (PEG) aqueous solutions were directly observed using fluorescence microscopy. The nanopore geometry was varied between circular and slit shapes, aspect ratios of 1:1, 1:5, and 1:20. A comparison across the overlap concentration of PEG revealed that above this concentration, DNA exhibits an elongation behavior immediately prior to entering the nanopore. Furthermore, the probability of clogging and the configurations of DNA during pore entry were analyzed, revealing a strong correlation between PEG concentration and DNA conformational dynamics near the nanopore.