The 71th Annual Meeting of JSFST
Aug 29 - Aug 31, 2024Meijo University
The 71th Annual Meeting of JSFST
Aug 29 - Aug 31, 2024Meijo University
[PA-42]Mechanisms underlying the vasorelaxant effects induced by 3-(4-hydroxy-3-methoxyphenyl)propionic acid and its conjugates in HUVECs
*Tint Ni Ni Tun1, Abe Chizumi1, Nishitani Yosuke2, Kayaki Hiroyuki2, Kawakami Hideaki2, Matsui Toshiro1,3(1. Grad. Sch. of Biores. & Bioenviro. Sci., Kyushu Univ., 2. Dept of Applied Bioscience group, Div of Development research & development, 3. Fac. of Agric., Grad. Sch. of Kyushu Univ)
【Purpose】 This study aims to investigate the potential physiological roles of 3-(4-hydroxy-3-methoxyphenyl) propionic acid (HMPA), and its metabolic forms, focusing on their ability to produce nitric oxide (NO), and their underlying molecular mechanisms in human umbilical vein endothelial cells (HUVECs).
【Methods】 The NO assay was performed by fluorometric NO assay kit ((Ex/Em: 355/460 nm). Cellular uptake and transport characteristics were investigated by using LC-QTOF/MS. Intracellular Ca2+ was detected with Calcium kit II-Fluo 4 by using Flex Station III multimode microplate reader (Ex/Em: 485/525 nm).
【Results】 HMPA and its conjugates (0.1 µM) stimulated NO production in HUVECs at 1 h. Transporter inhibitors for MCTs and OATPs significantly decreased the cellular uptake of all metabolites, while GLUT inhibitor only significantly reduced those of HMPA-GlcA and HMPA-S. HMPA and its conjugates significantly increased [Ca2+]i, IP3R inhibitor significantly abolished HMPA-GlcA-induced [Ca2+]i increase indicating glucuronidated HMPA promoted calcium release from the ER via the stimulation of IP3R receptor in HUVECs.
【Methods】 The NO assay was performed by fluorometric NO assay kit ((Ex/Em: 355/460 nm). Cellular uptake and transport characteristics were investigated by using LC-QTOF/MS. Intracellular Ca2+ was detected with Calcium kit II-Fluo 4 by using Flex Station III multimode microplate reader (Ex/Em: 485/525 nm).
【Results】 HMPA and its conjugates (0.1 µM) stimulated NO production in HUVECs at 1 h. Transporter inhibitors for MCTs and OATPs significantly decreased the cellular uptake of all metabolites, while GLUT inhibitor only significantly reduced those of HMPA-GlcA and HMPA-S. HMPA and its conjugates significantly increased [Ca2+]i, IP3R inhibitor significantly abolished HMPA-GlcA-induced [Ca2+]i increase indicating glucuronidated HMPA promoted calcium release from the ER via the stimulation of IP3R receptor in HUVECs.