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Holotomography and artificial intelligence:label-free 3D imaging, classification, and inferenceof live cells, tissues, and organoids

Until now, the conventional procedure for 3D observation of cells has been to first label cells with fluorescenc and then create sectioning images using a confocal microscope, but there have been concerns about artifacts on cells caused by fluorescent labeling and phototoxicity caused by fluorescent excitation. 　The label-free 3D imaging technology introduced in this seminar, as the name suggests, enables 3D observation without the need of fluorescent labeling. In addition, its extremely low phototoxicity makes it ideal for long time time-lapse observation of living cells. Recently, various cell analysis techniques have been developed using AI technology. In this seminar, we will introduce some of these AI analysis technologies as well.

State-of-the-Art, Label Free 3D Live Cell Imaging Microscopy

The 47th Annual Meeting of the Molecular Biology Society of Japan

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[3BT-04-01]Holotomography and artificial intelligence:label-free 3D imaging, classification, and inferenceof live cells, tissues, and organoids

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