Session Details
[3AS-16]【J】Understanding of biological and pathological events using proximity-dependent labeling technologies
Fri. Nov 29, 2024 9:00 AM - 11:15 AM JST
Fri. Nov 29, 2024 12:00 AM - 2:15 AM UTC
Fri. Nov 29, 2024 12:00 AM - 2:15 AM UTC
Room 16(Marine Messe Fukuoka Hall B, 2F Meeting Room 2-1)
Organizer: Kazuki Okuyama (RIKEN), Tetsuya Takano (Kyushu University)
Protein networks are essential for regulating a wide range of physiological, biological, and pathological events. Proximity-dependent labeling (PDL) technologies are recently emerged highly innovative proteomics approach, which contributes to the comprehensive analysis of protein functions in living cells or animals. In this session, we would like to introduce recent findings about molecular mechanisms underlying various biological events including infectious and neurological diseases utilizing PDL techniques. We will share the technical information with audience who plan to and wish to perform PDL for their further progress.
[3AS-16-01]In vivo BioID analysis with mice
○Kazuki Okuyama1, Ichiro Taniuchi1 (1. RIKEN IMS)
[3AS-16-02]Towards understanding the comprehensive molecular mechanisms of malaria parasite infection.
○Tomoko Ishino1, Naoaki Shinzawa1, Oundavong Sunti1, Rie Kubota1, Minami Baba1, Hidetaka Kosako2, Tatsuya Sawasaki3 (1. Tokyo Medical and Dental University, 2. Tokusima University , 3. Ehime University)
[3AS-16-03]Proximity-dependent labeling proteomics of nascent proteins
○Koshi Imami1 (1. RIKEN IMS)
[3AS-16-04]Protein degrader-dependent interaction analysis using a proximity-dependent biotinylation enzyme
○Satoshi Yamanaka1,2, Koya Nagaoka1, Yuki Shoya1, Kohei Nishino3, Hidetaka Kosako3, Tatsuya Sawasaki1 (1. Div. Cell-Free Sci., PROS, Ehime Univ., 2. Div. Proteo-interactome, PROS, Ehime Univ., 3. Inst. Adv. Med. Sci., Tokushima Univ.)
[3AS-16-05(2P-381)]Copper-free azido-DBCO click reaction labels and visualizes a group of biomolecules in living human cells.
○Takayuki Seki1, Hisato saitoh1,2 (1. Biology Course, Faculty of Science, Kumamoto University, 2. Faculty of Advanced Science and Technology (FAST), Kumamoto University)
[3AS-16-06(3P-452)]Novel positive regulator of mammalian Hedgehog signaling via phosphorylation of GLI2/GLI3
○Saishu Yoshida1,2, Akira Kawamura2, Katsuhiko Aoki3, Pattama Wiriyasermkul4,5, Shinya Sugimoto6,7,8, Junnosuke Tomiyoshi2, Ayasa Tajima4,9, Yamato Ishida10, Yohei Katoh10,11, Takehiro Tsukada1, Yousuke Tsuneoka12, Kohji Yamada2, Shushi Nagamori4,5, Kazuhisa Nakayama10, Kiyotsugu Yoshida2 (1. Dept. of Biomolecular Science, Toho Univ., 2. Dept. of Biochemistry, The Jikei Univ. School of Medicine, 3. Radioisotope Research Facilities, The Jikei Univ. School of Medicine, 4. Center for SI Medical Research, The Jikei Univ. School of Medicine, 5. Dept. of Laboratory Medicine, The Jikei Univ. School of Medicine, 6. Dept. of Bacteriology, The Jikei Univ. School of Medicine, 7. Center for Biofilm Science and Technology, The Jikei Univ. School of Medicine, 8. Laboratory of Amyloid Regulation, The Jikei Univ. School of Medicine, 9. Dept. of Molecular Biology, The Jikei Univ. School of Medicine , 10. Dept. of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto Univ., 11. Hiroshima Univ. Genome Editing Innovation Center, 12. Dept. of Anatomy, Faculty of Medicine, Toho Univ.)
[3AS-16-07]Exploration of promoting factors of tau aggregation using split-TurboID strategy in tauopathy model mouse in vivo
○Taeko Kimura1, Asahi Sakai1, Maria Kano1, Lisa Tatsumi1, Taisuke Tomita1 (1. Graduate school of pharmaceutical sciences, The University of Tokyo)
[3AS-16-08]Axon initial segments specific proteomics by antibody-directed extracellular proximity biotinylation
○Yuki Ogawa1,2, Matthew Rasband1 (1. Baylor College of Medicine, 2. University of South Carolina)
[3AS-16-09]Decoding Synaptic Proteome Dynamics Using BioID
○Tetsuya Takano1,2 (1. Kyushu University Institute for Advanced Study, Medical Institute of Bioregulation , 2. PRESTO, Japan Science and Technology Agency)