The 47th Annual Meeting of the Molecular Biology Society of Japan

Chair:Yohei Hanazaki(New England Biolabs Japan)

RNA sequencing is a method to study gene expression by comprehensively reading mRNA and ncRNA sequences using a next-generation sequencer. It has become a familiar method due to the improvement of sequencer functions and reduction of running costs. In addition, there are many choices of how to proceed, such as whether to perform library preparation and analysis by yourself or to ask a contracted service. In this seminar, we will introduce the advantages of preparing RNA libraries by yourself using the NEBNext RNA Library Preparation Kit, comparing them with the advantages of outsourcing. In addition, we will explain the key points to efficiently obtain reliable data, from sampling method to RNA purification, sequencing, and analysis. This seminar is full of useful information for both those who are just starting out and those who are already doing so.