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Wed. Dec 3, 2025

Thu. Dec 4, 2025

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Exploring the dynamic connectome with optical clearing and multicolor imaging

Brain functions are supported by dynamic changes in networks composed of various types of cells. To capture the dynamic nature of the connectome, we have developed optical clearing techniques and multicolor labeling/imaging methods for fluorescence microscopy. In this talk, I will introduce the following technologies: SeeDB2, which enables high-resolution structural imaging of fixed cells; SeeDB-Live, which allows for non-invasive clearing and live imaging of the living brain tissues; and fluorescent barcoding, which facilitates multiplexed labeling and imaging of neuronal circuits. Additionally, I will share critical tips for successfully applying these new technologies.

Evident Corporation

The 48th Annual Meeting of the Molecular Biology Society of Japan

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[2BT-15-02]Exploring the dynamic connectome with optical clearing and multicolor imaging

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