The 48th Annual Meeting of the Molecular Biology Society of Japan

Development and Application of Modification-specific Intracellular Antibody

Genetically encoded intracellular antibodies are useful for visualizing and manipulating endogenous proteins. However, the development of such probes has not been straightforward, as many single-chain antibodies suffer from issues with folding and stability. Recently, advances in AI-based protein design have made it possible to improve stability, thereby enabling more efficient development of intracellular antibodies. In this seminar, we will introduce the development and applications of intracellular antibodies that recognize post-translational modifications such as those of histones.