The 48th Annual Meeting of the Molecular Biology Society of Japan

The Key to Reliable IHC Data: Overcoming Reproducibility Challenges with Antibody Validation

Immunohistochemistry (IHC) is a powerful technique for analyzing protein expression in tissues, but achieving reliable and reproducible results can be a significant challenge. This issue is often linked to the quality of the antibodies used. This seminar will focus on antibody validation as the critical solution for improving IHC reproducibility. We'll explore why validation is so important and break down the detailed processes involved.

Drawing from the comprehensive, multi-faceted validation process practiced by Cell Signaling Technology (CST), we will walk through the specific steps you can take to obtain reliable data. This includes confirming antibody specificity using tissue arrays and cell pellets, as well as managing quality from lot to lot. By the end of this seminar, you will have the knowledge needed to confidently select high-quality antibodies and significantly enhance the reliability of your IHC data.