Presentation Information
[1410-24-12]Isolation of the new Mn(II)-oxidizing bacterial strain Pseudomonas sp. SK3 and its use in biotreatment of Mn(II)-containing wastewater
○Santisak Kitjanukit1, Naoko Okibe1 (1. Kyushu University)
司会：沖部 奈緒子（九州大学）
Keywords:
Mn(II)-containing wastewater,Mn(II)-oxidizing bacteria,Pseudomonas sp.
Manganese (Mn) in the form of Mn(II) can be readily oxidized at circumneutral pH enzymatically by Mn(II)-oxidizing microorganisms. This microbiological reaction could potentially enable more economically-feasible and environmentally-friendly operation for Mn(II) treatment process. Interestingly, such microbiological reaction was found to be responsible for natural attenuation of dissolved Mn(II) level and deposition of blacks Mn-oxides in a wastewater treatment plant. Our objective was set to isolate and characterize a robust Mn(II)-oxidizing microorganism with the aim to utilize it for treatment of actual Mn(II)-containing wastewaters. The new Mn(II)-oxidizing bacterium, strain SK3, was successfully isolated from the deposit (98% 16S rRNA gene sequence similarity with Pseudomonas resinovorans). Characteristics of strain SK3 were studied and compared with those of a well-studied Mn(II)-oxidizing Pseudomonas putida strain MnB1: the former showed more robust Mn(II) oxidation under various severe conditions. The results suggested that the new isolate SK3 might be useful for the actual wastewater treatment process.