Presentation Information
[P-38-05]Discovery of molecular signature of long-Term Psychiatric Sequelae of COVID-19 through Proteome profiling of dried blood spots
*Myungjae Baik1, Jong-Woo Paik1, Eun Young Kim2, Ah Rah Lee1, Jong Woo Kim1 (1.Kyung Hee University Hospital(Korea), 2.Seoul National University(Korea))
Keywords:
Proteomics,COVID-19,long COVID,Psychiatric Sequelae
Neuropsychiatric Sequelae are a major feature of post-acute sequelae of SARS-CoV-2 (PASC, known as long COVID), which has been major public health concern. Here, we aimed to identify molecular signatures reflecting PASC with psychiatric morbidities using dried blood spot (DBS) proteomic analysis. Study participants comprised 51 COVID-19 survivors ≧ 60 days after acute infection and categorized into three groups; clinically diagnosed with new onset psychiatric disorders (n=16, psychiatric PASC), persistent symptoms but without psychiatric disorders (n=18, general PASC), and symptomatically recovered (n=17, Recovered). Through The LC-MS/MS analysis, we identified a total of 1,604 proteins. Protein panel including Isoform 1 of Fibronectin, Sorbitol dehydrogenase, Cytosolic acyl coenzyme A thioester hydrolase, and Apolipoprotein A-II, could differentiate psychiatric PASC from recovered group at an AUC of 0.898 (95% CI: 0.7-1). Filamin-A and Vacuolar protein sorting-associated protein VTA1 homolog distinguished Psychiatric PASC groups from General PASC at an AUC of 0.823 (95% CI: 0.585-1). These proteins, which are involved in broad range of biological function such as cytoskeleton, cell membrane and immune response to viral infection, have suggested as potential biomarker of COVID 19 infection in recent literatures. Additional decision tree analysis showed that Alpha-synuclein, Pyruvate kinase PKM, and SORD performed well to distinguish three groups with an 82% classification accuracy. These proteins suggest that alteration of the immune, glucose and lipid metabolism pathways in response to SARS-CoV-2 infection and subsequent neuroinflammation and neurodegeneration were involved in clinical phenotype of psychiatric PASC. Our findings provide insights to potential molecular mechanisms of psychiatric PASC and suggest useful biomarkers for psychiatric disorders in the long-term clinical course of COVID-19.