Presentation Information
[P-6-02]Association between RNA Gene Expression Profiling and Cognitive Impairment in Ischaemic Stroke: A 3-month Prospective Study
*Suriati Mohamed Saini1,2, Nurul Nadiah Muhammad1, Katijjahbe Mohd. Ali2, Azian Abdul Murad1, Wan Asyraf Wan Zaidi1,2 (1.Universiti Kebangsaan Malaysia(Malaysia), 2.Hospital Canselor Tuanku Muhriz(Malaysia))
Keywords:
Cognitive Impairment,Stroke,Gene expression
Introduction: Stroke is a leading cause of long-term disability. Cognitive impairment is common but varies significantly among individuals. Genetics plays a crucial role in both the incidence and recovery from stroke. The objective of this study was to determine the association between targeted genes and cognitive impairment during an acute event of ischaemic stroke (IS) and at a 3-month follow-up.
Methods: We conducted a three-month prospective cohort study of adult patients with mild to moderate ischaemic stroke admitted to Hospital Canselor Tuanku Muhriz, Malaysia. The Montreal Cognitive Assessment (MoCA) was administered within one week of admission and at 3 months of follow-up to measure cognitive impairment. Peripheral whole blood of the individual with acute stroke patients and healthy controls was collected within one week of the stroke event for ribonucleic acid (RNA) extraction and analysis. Gene expression analysis was conducted using RT2 Profiler PCR arrays.
Results: A total of 24 stroke patients and 24 healthy controls were recruited in this study. Out of the ten RNA gene expression profiles examined in this study, the REST Corepressor 1 (RCOR1) and Phosphoinositide-3-Kinase Regulatory Subunit (PIK3R1) genes were shown to be significantly lower in stroke patients compared to healthy controls. The RCOR1 was significantly associated with cognitive impairment during acute stroke. The PIK3R1 was significantly associated with cognitive impairment at the 3-month follow-up.
Conclusion: This study found that RCOR1 and PIK3R genes were downregulated during an acute event of ischaemic stroke. Intriguingly, these genes were shown to be associated with cognitive impairment following ischaemic stroke. Further research with larger sample sizes is needed to validate these findings and refine these biomarkers for prognostic use in stroke.
Methods: We conducted a three-month prospective cohort study of adult patients with mild to moderate ischaemic stroke admitted to Hospital Canselor Tuanku Muhriz, Malaysia. The Montreal Cognitive Assessment (MoCA) was administered within one week of admission and at 3 months of follow-up to measure cognitive impairment. Peripheral whole blood of the individual with acute stroke patients and healthy controls was collected within one week of the stroke event for ribonucleic acid (RNA) extraction and analysis. Gene expression analysis was conducted using RT2 Profiler PCR arrays.
Results: A total of 24 stroke patients and 24 healthy controls were recruited in this study. Out of the ten RNA gene expression profiles examined in this study, the REST Corepressor 1 (RCOR1) and Phosphoinositide-3-Kinase Regulatory Subunit (PIK3R1) genes were shown to be significantly lower in stroke patients compared to healthy controls. The RCOR1 was significantly associated with cognitive impairment during acute stroke. The PIK3R1 was significantly associated with cognitive impairment at the 3-month follow-up.
Conclusion: This study found that RCOR1 and PIK3R genes were downregulated during an acute event of ischaemic stroke. Intriguingly, these genes were shown to be associated with cognitive impairment following ischaemic stroke. Further research with larger sample sizes is needed to validate these findings and refine these biomarkers for prognostic use in stroke.