第16回バイオ関連化学シンポジウム
2022年9月10日〜9月12日ハイブリッド開催（名古屋大学／オンライン）
第16回バイオ関連化学シンポジウム
2022年9月10日〜9月12日ハイブリッド開催（名古屋大学／オンライン）
[1B-06]Effect of Hydrophobic Aromatic Group on Amphiphilic Oligo(ethylene glycol)s as Lysozyme Stabilizers
*Wijak Yospanya1, Kohei Sato1, Kazushi Kinbara1(1. School of Life Science and Technology, Tokyo Institute of Technology)
High-efficiency monodisperse protein stabilizers are essential to expanding the applications of proteins and enzymes. We reported the successful lysozyme heat-induced aggregation suppression using phenyl-appending octa(ethylene glycol), demonstrating the necessity of amphiphilicity and minimum length of oligo(ethylene glycol) in the design of protein stabilizer. Here, we will discuss the effect of hydrophobic aromatic groups: naphthalene and triptycene. Unexpectedly, naphthalene shows superior suppression efficiency at significantly lower ratio than phenyl group. In addition, the length of oligo(ethylene glycol) does not clearly affect the stabilization efficiency. Therefore, there are still several unknown key factors to the optimal designs of amphiphilic oligo(ethylene glycol) stabilizers.