講演情報
[P-86（E）]Assessment of the mechanical and biological behavior of tissue conditioner developed with cetylpyridinium chloride and nanoporous silica
*Sirus Safaee1, Hiroshi Murata1 (1. Department of Prosthetic Dentistry, Graduate School of Biomedical Sciences, Nagasaki University)
[Purpose]
Tissue conditioners are flexible materials used in prosthetic dentistry to line denture surfaces, enhancing comfort and supporting oral tissues.1) A major challenge is the colonization of Candida albicans, which can lead to infections and discomfort. This study hypothesizes that a tissue conditioner with drug-release properties can prevent microbial infections. Nano-porous silica (NPS) serves as a carrier for therapeutic agents, while cetylpyridinium chloride (CPC) acts as an antibacterial agent. Our goal is to develop a novel tissue conditioner that integrates these technologies to improve functionality, durability, and patient comfort.
[Method]
Two sample types were created: one using a stainless-steel mold for mechanical properties and another with a silicone mold for drug delivery assessment. Samples were stored in water at 37°C and evaluated for viscoelastic properties over time, both before and after incorporating CPC-loaded NPS. We compared commercial Shofu tissue conditioner II (STC) with our developed drug-loaded tissue conditioner, STC-NPS. Dynamic viscoelastic properties were measured using a controlled-stress rheometer (Discovery HR-2, TA Instruments, New Castle, DE, USA). Statistical analyses included one-way ANOVA and Tukey and T-test performed at p<0.05. Drug-loading involved using NPS as a carrier, with CPC incorporated via dip-immersion and freeze-drying. Samples were exposed to Candida albicans for two weeks at 37°C and analyzed using scanning electron microscopy.
[Results and Discussion]
The gelation time of STC-NPS was slightly reduced compared to STC. Viscoelastic properties showed no significant differences between groups, indicating that NPS incorporation does not compromise mechanical integrity. Scanning electron microscopy confirmed CPC's efficacy in suppressing Candida albicans colonization, demonstrating a significant reduction in microbial presence on STC-NPS compared to STC. These findings suggest that NPS-CPC enhances tissue conditioners, offering improved infection prevention, effective healing, and increased patient comfort in prosthodontics. This innovative approach represents a significant advancement in oral health care, paving the way for better patient outcomes.
[References]
1) Murata H, Chimori H, Hong G, et al. Compatibility of tissue conditioners and denture cleansers: Influence on surface conditions. Dent Mater J 2010;29:446-453.
Tissue conditioners are flexible materials used in prosthetic dentistry to line denture surfaces, enhancing comfort and supporting oral tissues.1) A major challenge is the colonization of Candida albicans, which can lead to infections and discomfort. This study hypothesizes that a tissue conditioner with drug-release properties can prevent microbial infections. Nano-porous silica (NPS) serves as a carrier for therapeutic agents, while cetylpyridinium chloride (CPC) acts as an antibacterial agent. Our goal is to develop a novel tissue conditioner that integrates these technologies to improve functionality, durability, and patient comfort.
[Method]
Two sample types were created: one using a stainless-steel mold for mechanical properties and another with a silicone mold for drug delivery assessment. Samples were stored in water at 37°C and evaluated for viscoelastic properties over time, both before and after incorporating CPC-loaded NPS. We compared commercial Shofu tissue conditioner II (STC) with our developed drug-loaded tissue conditioner, STC-NPS. Dynamic viscoelastic properties were measured using a controlled-stress rheometer (Discovery HR-2, TA Instruments, New Castle, DE, USA). Statistical analyses included one-way ANOVA and Tukey and T-test performed at p<0.05. Drug-loading involved using NPS as a carrier, with CPC incorporated via dip-immersion and freeze-drying. Samples were exposed to Candida albicans for two weeks at 37°C and analyzed using scanning electron microscopy.
[Results and Discussion]
The gelation time of STC-NPS was slightly reduced compared to STC. Viscoelastic properties showed no significant differences between groups, indicating that NPS incorporation does not compromise mechanical integrity. Scanning electron microscopy confirmed CPC's efficacy in suppressing Candida albicans colonization, demonstrating a significant reduction in microbial presence on STC-NPS compared to STC. These findings suggest that NPS-CPC enhances tissue conditioners, offering improved infection prevention, effective healing, and increased patient comfort in prosthodontics. This innovative approach represents a significant advancement in oral health care, paving the way for better patient outcomes.
[References]
1) Murata H, Chimori H, Hong G, et al. Compatibility of tissue conditioners and denture cleansers: Influence on surface conditions. Dent Mater J 2010;29:446-453.
