講演情報
[18p-C32-9]Development of nanoforce biosensors operating in liquids and tailoring their properties by integrating additives.
〇(D)Fabiano Altieri1, Kaori Sugihara1 (1.IIS for Todai)
キーワード：
Polydiacetylene、Atomic Force Microscope、Nano-Friction Force Microscope
The COVID-19 pandemic created rapid and low-cost diagnostic tools like the rapid antigen detection test (RADT) for SARS-CoV-2, using saliva or nasal samples. Although less accurate than RT-PCR tests, RADTs have been crucial for virus control but are disposable and environmentally harmful, offering only qualitative results. This study presents a potential RADT using 10,12-tricosadiynoic acid (TRCDA) and biological lipids to develop a polydiacetylene (PDA)-based biosensor. This sensor is tunable, cost-effective, fast, and user-friendly for detecting any potential physical and biochemical agent. TRCDA polymerizes into a mechanochromic PDA polymer that changes color and fluorescence under stress. Unlike previous qualitative uses, this study quantifies the force-fluorescence relationship at the nanoscale using Nano-Friction Force Microscopy (NFFM) and fluorescence microscopy to measure target agent concentration in a solution. Integrating phosphatidylcholine lipids with TRCDA enhances sensitivity, and chemical modifications aim to make the sensor electronically reversible and reusable.
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