講演情報
[10a-A13-4]Investigating the physiology and dynamics of tumor spheroids by dynamic optical-coherence microscopy and fluorescence microscopy
〇(D)Rion Morishita1, Ibrahim Abd El-Sadek1,2, Atsuko Furukawa3, Satoshi Matsusaka3, Shuichi Makita1, Yoshiaki Yasuno1 (1.COG, Univ. of Tsukuba, 2.Damietta Univ., 3.Medicine, Univ. of Tsukuba)
キーワード：
Optical coherence tomography、Dynamic OCT、spheroid
This paper investigates the relationship between intracellular dynamics in tumor spheroids visualized by dynamic optical coherence tomography (DOCT) and the physiological cell status determined via fluorescence (FLUO) microscopy. Our results demonstrate that the periphery of MCF-7 spheroids shows a FLUO signal, indicating “highly metabolic cytoplasm”, whereas the spheroid core shows a FLUO signal, indicating “nuclei under necrosis or late apoptosis.” Correspondingly, DOCT reveals that the spheroid periphery consists of “a high occupancy of dynamic scatterers moving at low speeds,” whereas the spheroid core consists of “a low occupancy of dynamic scatterers moving at high speeds.”