講演情報
[10a-PA6-9]Intracellular Thermometry During Cellular Freezing Using Nitrogen-Vacancy Centers in Nanodiamonds
〇Pawan Kumar1, Komal Raje1, Shogo Oe1, Kunitaka Hayashi1, Masaru Nakada2, Toshu An1, Kazuaki Matsumura1 (1.JAIST, 2.Toray Research Center, Inc)
キーワード：
Intracellular quantum thermometry、NV center、Nanodiamond
Intracellular thermometry during freezing is important for cryobiology and cryopreservation, and NV-center fluorescent nanodiamonds provide a promising sensor platform due to their photostability, biocompatibility, and temperature sensitivity. Here, we develop a confocal thermometry platform based on NV nanodiamond optically detected magnetic resonance (ODMR) microscopy integrated with a cooling stage and demonstrate quantitative temperature measurements in frozen cells. Temperature was determined from the zero-field splitting of NV-center ODMR spectra. Intracellular 100 nm nanodiamonds maintained stable optical and spin properties after freezing, exhibiting photoluminescence count rates of 1 million counts per sec and ODMR contrast of 4–5 percent at 532 nm excitation below 1 mW. ODMR spectra recorded from 20 degC to −40 degC confirmed preservation of NV-center spin properties, establishing NV nanodiamond thermometry for thermal monitoring of frozen biological systems.