講演情報
[8p-C214-9]Direct Molecular Delivery into Escherichia coli Using Gold Nanotubes for Synthetic Biology Applications
〇(DC)DaniellaMarie Beltran Gatus1, Kazuhiro Oyama1, Sally Wang2, Chen-Yu Tsao2, Monica Chu2, William E. Bentley2, Saman Azhari1, Gabor Memes1, Takeo Miyake1 (1.Waseda Univ., 2.Univ. of Maryland)
キーワード：
synthetic biology、gold nanotube、microbiology
Microorganisms are vital to biotechnology, food-and pharmaceuticals production, and the synthesis of bioactive compounds. Efficient delivery of exogenous genes and functional molecules into microbial cells is critical for harnessing and engineering their capabilities. However, conventional transformation methods, such as chemical transformation and electroporation, often suffer from limitations in delivery efficiency and applicability across different microbial species. In this study, we demonstrate the use of 200-nm diameter gold nanotubes (AuNTs) for direct cargo delivery into Escherichia coli (E. coli, Figure 1a).
The AuNTs can deliver a wide variety of molecules, including calcein, a fluorescence dye, as shown via the comparison between the control and injected groups in Figure 1b and c, respectively. Moreover, plasmid delivery was also tested by injecting YTK001, a plasmid with chloramphenicol (Cm50) antibiotic resistance and constitutive superfolder green fluorescence protein (sfGFP) expression. After delivery, the sample was spread onto Cm50 selective agar plates to confirm successful plasmid delivery, which is seen in Figure 1d and e from the colonies formed from the sample in contrast to the control group. This was further validated by imaging fluorescence from sfGFP, recorded from a colony of the sample group that was picked and grown in medium overnight.
These results demonstrate that 200-nm diameter AuNTs can effectively deliver molecules of varying dimensions and composition directly into E. coli, highlighting the potential of AuNT-based injection as a promising transformation method for synthetic biology and genetic engineering applications.
Reference: B. Liu, Z. Dai, B. Zhang, K. Oyama, C. Li, Y. Chen, M. Cui, T. Miyake, Small Science2026, 6, e202500598.
The AuNTs can deliver a wide variety of molecules, including calcein, a fluorescence dye, as shown via the comparison between the control and injected groups in Figure 1b and c, respectively. Moreover, plasmid delivery was also tested by injecting YTK001, a plasmid with chloramphenicol (Cm50) antibiotic resistance and constitutive superfolder green fluorescence protein (sfGFP) expression. After delivery, the sample was spread onto Cm50 selective agar plates to confirm successful plasmid delivery, which is seen in Figure 1d and e from the colonies formed from the sample in contrast to the control group. This was further validated by imaging fluorescence from sfGFP, recorded from a colony of the sample group that was picked and grown in medium overnight.
These results demonstrate that 200-nm diameter AuNTs can effectively deliver molecules of varying dimensions and composition directly into E. coli, highlighting the potential of AuNT-based injection as a promising transformation method for synthetic biology and genetic engineering applications.
Reference: B. Liu, Z. Dai, B. Zhang, K. Oyama, C. Li, Y. Chen, M. Cui, T. Miyake, Small Science2026, 6, e202500598.